| IED ID | IndEnz0002001342 |
| Enzyme Type ID | protease001342 |
| Protein Name |
Extracellular small neutral protease EC 3.4.24.77 Extracellular metalloprotease Snapalysin |
| Gene Name | snpA lmp mprA mprA2 prt SCO7432 SC6D11.28c |
| Organism | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces albidoflavus group Streptomyces coelicolor Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) |
| Enzyme Sequence | MRITLPLLSTAVGLGLTAAVLGTGPAATAAAPQEPVRAAQLGYQPSAGSGEDAAANRAFFEAVVKSVAEKRAANPSAAAAVTVYYSATNAPSFRSQISRSAQIWNSSVSNVRLAESSSGADFAYYEGNDSRGSYASTDGHGSGYIFLDYRQNQQYDSTRVTAHETGHVLGLPDHYSGPCSELMSGGGPGPSCTNPYPNSTERSRVNQLWAYGFQAALDKALEKASQR |
| Enzyme Length | 227 |
| Uniprot Accession Number | P0A3Z7 |
| Absorption | |
| Active Site | ACT_SITE 164; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolyzes proteins with a preference for Tyr or Phe in the P1' position. Has no action on amino-acid p-nitroanilides.; EC=3.4.24.77; |
| DNA Binding | |
| EC Number | 3.4.24.77 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (1); Metal binding (5); Propeptide (1); Signal peptide (1) |
| Keywords | Calcium;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | PTM: The N-terminus is blocked. {ECO:0000250}. |
| Signal Peptide | SIGNAL 1..29; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 23,765 |
| Kinetics | |
| Metal Binding | METAL 156; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P56406; METAL 158; /note=Calcium; /evidence=ECO:0000250|UniProtKB:P56406; METAL 163; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406; METAL 167; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406; METAL 173; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P56406 |
| Rhea ID | |
| Cross Reference Brenda |