| IED ID | IndEnz0002001351 |
| Enzyme Type ID | protease001351 |
| Protein Name |
ECF RNA polymerase sigma factor SigL ECF sigma factor SigL Alternative RNA polymerase sigma factor SigL RNA polymerase sigma-L factor Sigma-L factor |
| Gene Name | sigL Rv0735 |
| Organism | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Corynebacteriales Mycobacteriaceae Mycobacterium Mycobacterium tuberculosis complex Mycobacterium tuberculosis Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
| Enzyme Sequence | MARVSGAAAAEAALMRALYDEHAAVLWRYALRLTGDAAQAEDVVQETLLRAWQHPEVIGDTARPARAWLFTVARNMIIDERRSARFRNVVGSTDQSGTPEQSTPDEVNAALDRLLIADALAQLSAEHRAVIQRSYYRGWSTAQIATDLGIAEGTVKSRLHYAVRALRLTLQELGVTR |
| Enzyme Length | 177 |
| Uniprot Accession Number | P9WGH5 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | DNA_BIND 141..160; /note=H-T-H motif; /evidence=ECO:0000255 |
| EC Number | |
| Enzyme Function | FUNCTION: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis. Over-expression of SigL induces 19-28 genes including polyketide synthases, secreted and membrane proteins. Might play a minor role in regulating SigB. {ECO:0000269|PubMed:16199577, ECO:0000269|PubMed:16552079}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (2); Chain (1); DNA binding (1); Helix (9); Motif (1); Region (2) |
| Keywords | 3D-structure;DNA-binding;Reference proteome;Sigma factor;Transcription;Transcription regulation;Virulence |
| Interact With | |
| Induction | INDUCTION: Expressed during growth in culture; expression increases from lag to exponential phase. Has a weak SigL-independent promoter, is also weakly autoregulated, no other sigma factor allows its transcription. Forms an operon with rslA. {ECO:0000269|PubMed:16199577, ECO:0000269|PubMed:16552079}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (9) |
| Cross Reference PDB | 3HUG; 6DV9; 6DVB; 6DVC; 6DVD; 6DVE; 6TYE; 6TYF; 6TYG; |
| Mapped Pubmed ID | 20124702; 22505256; 30755604; 32127479; |
| Motif | MOTIF 42..45; /note=Interaction with polymerase core subunit RpoC; /evidence=ECO:0000250 |
| Gene Encoded By | |
| Mass | 19,538 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |