Detail Information for IndEnz0002001361
IED ID IndEnz0002001361
Enzyme Type ID protease001361
Protein Name Transmembrane emp24 domain-containing protein 2
Membrane protein p24A
p24
p24 family protein beta-1
p24beta1
Gene Name TMED2 RNP24
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MVTLAELLVLLAALLATVSGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV
Enzyme Length 201
Uniprot Accession Number Q15363
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase-activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing. {ECO:0000269|PubMed:10761932, ECO:0000269|PubMed:17693410, ECO:0000269|PubMed:20361938, ECO:0000269|PubMed:20427317, ECO:0000269|PubMed:21219331}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (8); Chain (1); Coiled coil (1); Domain (1); Motif (2); Mutagenesis (4); Region (2); Signal peptide (1); Topological domain (2); Transmembrane (1)
Keywords 3D-structure;Coiled coil;Cytoplasmic vesicle;ER-Golgi transport;Endoplasmic reticulum;Golgi apparatus;Membrane;Protein transport;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport
Interact With P41180; P13987; P55085; P49755; O35587
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000255}. Cytoplasmic vesicle, COPI-coated vesicle membrane {ECO:0000269|PubMed:9472029}; Single-pass type I membrane protein {ECO:0000255}. Golgi apparatus, cis-Golgi network membrane {ECO:0000269|PubMed:10852829, ECO:0000269|PubMed:12237308}; Single-pass type I membrane protein {ECO:0000255}. Golgi apparatus, Golgi stack membrane {ECO:0000269|PubMed:10852829, ECO:0000269|PubMed:12237308}; Single-pass type I membrane protein {ECO:0000255}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:10852829, ECO:0000269|PubMed:12237308}; Single-pass type I membrane protein {ECO:0000255}. Endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000269|PubMed:12237308}; Single-pass type I membrane protein {ECO:0000255}. Note=Cycles between compartments of the early secretatory pathway. {ECO:0000269|PubMed:12237308}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000255
Structure 3D X-ray crystallography (1)
Cross Reference PDB 5AZW;
Mapped Pubmed ID 10052452; 10214941; 10366590; 10679020; 10747087; 10825291; 10903204; 10935626; 10982397; 11035026; 11035033; 11135303; 11172815; 11285137; 11306556; 11461920; 11493604; 11689559; 11703931; 11726511; 11739780; 11748249; 11927603; 12235121; 12388752; 14504276; 14654841; 14742712; 15029241; 15272311; 15632110; 15728195; 15878873; 16303754; 16571679; 16624868; 16633337; 16641999; 16723730; 17005010; 17038314; 17113389; 17192411; 17253781; 17274799; 17287728; 17316621; 17760859; 17927562; 18086915; 18182008; 18809720; 18843296; 19015319; 19109418; 19151722; 19164740; 19296914; 19369418; 19566487; 19631211; 19638414; 19656848; 19758564; 20005805; 20136777; 20360068; 20462495; 20534429; 20545907; 20637885; 20679433; 20711500; 20966969; 21478858; 21532587; 21550981; 21911578; 21988832; 22013193; 22094269; 22157747; 22190034; 22212250; 22675024; 23378591; 23396477; 23455153; 23479643; 23698585; 23839779; 24119662; 24189400; 24806965; 24917561; 25364732; 25406594; 25436559; 25586378; 25619252; 26496610; 26638075; 30540941; 32293333; 7691276; 7844144; 8001155; 8128252; 8253837; 8636227; 8663406; 8991093; 9094723; 9150144; 9244302; 9244307; 9288971; 9307973; 9323141; 9380700; 9382863; 9405360; 9490716; 9647643; 9671725; 9751720; 9990005;
Motif MOTIF 194..201; /note=COPI vesicle coat-binding; /evidence=ECO:0000255; MOTIF 194..195; /note=COPII vesicle coat-binding; /evidence=ECO:0000255
Gene Encoded By
Mass 22,761
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda