Detail Information for IndEnz0002001370
IED ID IndEnz0002001370
Enzyme Type ID protease001370
Protein Name Tripeptidyl-peptidase 1
TPP-1
EC 3.4.14.9
Lysosomal pepstatin-insensitive protease
LPIC
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase I
TPP-I
Gene Name Tpp1 Cln2
Organism Mus musculus (Mouse)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse)
Enzyme Sequence MGLQARLLGLLALVIAGKCTYNPEPDQRWMLPPGWVSLGRVDPEEELSLTFALKQRNLERLSELVQAVSDPSSPQYGKYLTLEDVAELVQPSPLTLLTVQKWLSAAGARNCDSVTTQDFLTCWLSVRQAELLLPGAEFHRYVGGPTKTHVIRSPHPYQLPQALAPHVDFVGGLHRFPPSSPRQRPEPQQVGTVSLHLGVTPSVLRQRYNLTAKDVGSGTTNNSQACAQFLEQYFHNSDLTEFMRLFGGSFTHQASVAKVVGKQGRGRAGIEASLDVEYLMSAGANISTWVYSSPGRHEAQEPFLQWLLLLSNESSLPHVHTVSYGDDEDSLSSIYIQRVNTEFMKAAARGLTLLFASGDTGAGCWSVSGRHKFRPSFPASSPYVTTVGGTSFKNPFLITDEVVDYISGGGFSNVFPRPPYQEEAVAQFLKSSSHLPPSSYFNASGRAYPDVAALSDGYWVVSNMVPIPWVSGTSASTPVFGGILSLINEHRILNGRPPLGFLNPRLYQQHGTGLFDVTHGCHESCLNEEVEGQGFCSGPGWDPVTGWGTPNFPALLKTLLNP
Enzyme Length 562
Uniprot Accession Number O89023
Absorption
Active Site ACT_SITE 271; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:O14773; ACT_SITE 275; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:O14773; ACT_SITE 474; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:O14773
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of an N-terminal tripeptide from a polypeptide, but also has endopeptidase activity.; EC=3.4.14.9; Evidence={ECO:0000269|PubMed:28464005};
DNA Binding
EC Number 3.4.14.9
Enzyme Function FUNCTION: Lysosomal serine protease with tripeptidyl-peptidase I activity (PubMed:28464005). May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases (By similarity). Requires substrates with an unsubstituted N-terminus (By similarity). {ECO:0000250|UniProtKB:O14773, ECO:0000269|PubMed:28464005}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (3); Domain (1); Erroneous initiation (1); Glycosylation (5); Metal binding (5); Mutagenesis (1); Propeptide (1); Sequence conflict (2); Signal peptide (1)
Keywords Autocatalytic cleavage;Calcium;Disulfide bond;Glycoprotein;Hydrolase;Lysosome;Metal-binding;Protease;Reference proteome;Serine protease;Signal;Zymogen
Interact With Q91WC1
Induction
Subcellular Location SUBCELLULAR LOCATION: Lysosome {ECO:0000269|PubMed:19941651}. Melanosome {ECO:0000250|UniProtKB:O14773}.
Modified Residue
Post Translational Modification PTM: Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity (By similarity). {ECO:0000250|UniProtKB:O14773}.
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000250|UniProtKB:O14773
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10330339; 10527801; 11217851; 11423996; 12038963; 12466851; 14609438; 14651853; 15158442; 15483130; 16602821; 16615898; 17053789; 17948054; 18343701; 18570628; 18639872; 19429009; 19749771; 19995905; 20231318; 20493811; 21267068; 21996941; 22099311; 22101936; 22792360; 22989886; 23804102; 25056954; 25303899; 25917818; 26778124; 27101989; 28728022; 29408933; 30022158; 30928643;
Motif
Gene Encoded By
Mass 61,342
Kinetics
Metal Binding METAL 516; /note=Calcium; /evidence=ECO:0000250|UniProtKB:O14773; METAL 517; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:O14773; METAL 538; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:O14773; METAL 540; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:O14773; METAL 542; /note=Calcium; /evidence=ECO:0000250|UniProtKB:O14773
Rhea ID
Cross Reference Brenda 3.4.14.9;