| IED ID | IndEnz0002001383 |
| Enzyme Type ID | protease001383 |
| Protein Name |
Transmembrane emp24 domain-containing protein 10 Protein TMED10 21 kDa transmembrane-trafficking protein S31I125 S31III125 Tmp-21-I Transmembrane protein Tmp21 p23 p24 family protein delta-1 p24delta1 p24delta |
| Gene Name | TMED10 TMP21 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MSGLSGPPARRGPFPLALLLLFLLGPRLVLAISFHLPINSRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLKITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSMFCLIGLATWQVFYLRRFFKAKKLIE |
| Enzyme Length | 219 |
| Uniprot Accession Number | P49755 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Cargo receptor involved in protein vesicular trafficking and quality control in the endoplasmic reticulum (ER) and Golgi (PubMed:10052452, PubMed:11726511, PubMed:16641999, PubMed:17288597, PubMed:19296914, PubMed:20427317, PubMed:21219331, PubMed:27569046). The p24 protein family is a group of transmembrane proteins that bind coat protein complex I/COPI and coat protein complex II/COPII involved in vesicular trafficking between the membranes (PubMed:10052452). Acts at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and involved in vesicle coat formation at the cytoplasmic side (PubMed:20427317, PubMed:27569046). Mainly functions in the early secretory pathway and cycles between the ER, ER-Golgi intermediate compartment (ERGIC) and Golgi, mediating cargo transport through COPI and COPII-coated vesicles (PubMed:10052452, PubMed:10852829, PubMed:12237308). In COPII vesicle-mediated anterograde transport, involved in the transport of GPI-anchored proteins by acting together with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER (PubMed:20427317, PubMed:27569046). Recognizes GPI anchors structural remodeled in the ER by the GPI inositol-deacylase/PGAP1 and the metallophosphoesterase MPPE1/PGAP5 (By similarity). In COPI vesicle-mediated retrograde transport, involved in the biogenesis of COPI vesicles and vesicle coat recruitment (PubMed:11726511). Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent from the modulation of gamma-secretase activity) (PubMed:17288597). Involved in the KDELR2-mediated retrograde transport of the toxin A subunit (CTX-A-K63)together with COPI and the COOH terminus of KDELR2 (By similarity). On Golgi membranes, acts as primary receptor for ARF1-GDP, a GTP-binding protein involved in COPI-vesicle formation (PubMed:11726511). Increases coatomer-dependent GTPase-activating activity of ARFGAP2 which mediates the hydrolysis of ARF1-bound GTP and therefore modulates protein trafficking from the Golgi apparatus (PubMed:19296914). Involved in the exocytic trafficking of G protein-coupled receptors F2LR1/PAR2 (trypsin and tryspin-like enzyme receptor), OPRM1 (opioid receptor) and P2RY4 (UTD and UDP receptor) from the Golgi to the plasma membrane, thus contributing to receptor resensitization (PubMed:21219331). In addition to its cargo receptor activity, may also act as a protein channel after oligomerization, facilitating the post-translational entry of leaderless cytoplasmic cargo into the ERGIC (PubMed:32272059). Involved in the translocation into ERGIC, the vesicle entry and the secretion of leaderless cargos (lacking the secretion signal sequence), including the mature form of interleukin 1/IL-1 family members, the alpha-crystallin B chain HSPB5, the carbohydrate-binding proteins galectin-1/LGALS1 and galectin-3/LGALS3, the microtubule-associated protein Tau/MAPT, and the annexin A1/ANXA1; the translocation process is dependent on cargo protein unfolding and enhanced by chaperones HSP90AB1 and HSP90B1/GRP9 (PubMed:32272059). Could also associates with the presenilin-dependent gamma-secretase complex in order to regulate gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40/Abeta40 (PubMed:16641999). {ECO:0000250|UniProtKB:Q28735, ECO:0000250|UniProtKB:Q63584, ECO:0000269|PubMed:10052452, ECO:0000269|PubMed:10852829, ECO:0000269|PubMed:11726511, ECO:0000269|PubMed:12237308, ECO:0000269|PubMed:16641999, ECO:0000269|PubMed:17288597, ECO:0000269|PubMed:19296914, ECO:0000269|PubMed:20427317, ECO:0000269|PubMed:21219331, ECO:0000269|PubMed:27569046, ECO:0000269|PubMed:32272059, ECO:0000303|PubMed:10052452}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Erroneous initiation (1); Frameshift (1); Glycosylation (1); Modified residue (2); Motif (2); Mutagenesis (4); Natural variant (2); Region (4); Sequence conflict (1); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Cell membrane;Cytoplasmic vesicle;Direct protein sequencing;ER-Golgi transport;Endoplasmic reticulum;Glycoprotein;Golgi apparatus;Membrane;Methylation;Protein transport;Reference proteome;Signal;Transmembrane;Transmembrane helix;Transport |
| Interact With | P05067; Q8WXH2; Q92542; P49768; Q9NZ42; P17706-1; Q15363; Q8XAL7 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:10852829, ECO:0000269|PubMed:12237308, ECO:0000269|PubMed:9472029}; Single-pass type I membrane protein {ECO:0000255}. Endoplasmic reticulum-Golgi intermediate compartment membrane {ECO:0000269|PubMed:12237308, ECO:0000269|PubMed:32272059, ECO:0000269|PubMed:9382861}; Single-pass type I membrane protein {ECO:0000255}. Golgi apparatus membrane {ECO:0000269|PubMed:10852829, ECO:0000269|PubMed:12237308, ECO:0000269|PubMed:21219331, ECO:0000269|PubMed:9382861, ECO:0000269|PubMed:9472029}; Single-pass type I membrane protein {ECO:0000255}. Golgi apparatus, cis-Golgi network membrane {ECO:0000269|PubMed:9382861}; Single-pass type I membrane protein {ECO:0000255}. Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:Q63584}; Single-pass type I membrane protein {ECO:0000255}. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000255}. Cell membrane {ECO:0000250|UniProtKB:Q63584}; Single-pass type I membrane protein {ECO:0000255}. Melanosome {ECO:0000269|PubMed:17081065}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. {ECO:0000269|PubMed:17081065}. |
| Modified Residue | MOD_RES 171; /note=Dimethylated arginine; /evidence=ECO:0000250|UniProtKB:Q63584; MOD_RES 176; /note=Dimethylated arginine; /evidence=ECO:0000250|UniProtKB:Q63584 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..31; /evidence="ECO:0000269|PubMed:19892738, ECO:0007744|PubMed:25944712" |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10679020; 10747087; 10825291; 10903204; 10982397; 11035026; 11035033; 11172815; 11285137; 11306556; 11461920; 11493604; 11689559; 11703931; 11739780; 11748249; 11927603; 12235121; 12388752; 14504276; 14654841; 14742712; 15029241; 15272311; 15632110; 15728195; 15878873; 16303754; 16571679; 16595549; 16624868; 16633337; 16723730; 17005010; 17038314; 17113389; 17192411; 17253781; 17274799; 17287728; 17316621; 17353931; 17760859; 17927562; 18086915; 18182008; 18405662; 18652896; 18809720; 18843296; 19015319; 19046380; 19109418; 19151722; 19164740; 19343178; 19369418; 19566487; 19631211; 19638414; 19656848; 19710022; 19758564; 19805454; 20005805; 20136777; 20164256; 20462495; 20534429; 20545907; 20546612; 20562859; 20637885; 20679433; 20966969; 21454541; 21478858; 21532587; 21550981; 21699748; 21911578; 21988832; 22013193; 22094269; 22157747; 22190034; 22204304; 22299712; 22304920; 22575643; 22675024; 23378591; 23396477; 23416715; 23434013; 23455153; 23479643; 23698585; 23839779; 24119662; 24189400; 24806965; 24917561; 25364732; 25406594; 25436559; 25586378; 25609649; 25619252; 26496610; 26617795; 26638075; 26752685; 28115518; 28233271; 30821607; 30996093; 31472964; 32293333; 7691276; 7844144; 8001155; 8128252; 8253837; 8636227; 8663406; 8991093; 9094723; 9150144; 9244307; 9288971; 9307973; 9323141; 9380700; 9382863; 9405360; 9490716; 9647643; 9671725; 9751720; 9990005; |
| Motif | MOTIF 211..219; /note=COPI vesicle coat-binding; MOTIF 211..212; /note=COPII vesicle coat-binding |
| Gene Encoded By | |
| Mass | 24,976 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |