Detail Information for IndEnz0002001387
IED ID IndEnz0002001387
Enzyme Type ID protease001387
Protein Name Tail tip assembly protein K
Gene product 19
gp19
Probable endopeptidase
EC 3.4.-.-
Tail assembly protein K
Gene Name gene 19
Organism Escherichia phage N15 (Bacteriophage N15)
Taxonomic Lineage Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes Caudovirales Siphoviridae (phages with long non-contractile tails) Ravinvirus Escherichia phage N15 (Bacteriophage N15)
Enzyme Sequence MRQKTIDAIMAHAAAEYPRECCGVVAQKSRVERYFPCRNLSAEPTEHFHLSPEDYAAAEDWGTVVAIVHSHPDATTQPSELDKAQCDATLLPWHIVSWPEGDLRTIQPRGELPLLERPFVLGHFDCWGLVMSYFRQTHGIELHDYRVDYPWWENSYPDNFYQDCWYECGFREFDGPPQEGDLVIMQVQADKWNHAGILLEGNMLLHHLYGHLSQRVPYGGYWQERTMKIVRYKDVRGNEACRK
Enzyme Length 243
Uniprot Accession Number O64333
Absorption
Active Site ACT_SITE 126; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284; ACT_SITE 194; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284; ACT_SITE 206; /evidence=ECO:0000255|PROSITE-ProRule:PRU01284
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.-.-
Enzyme Function FUNCTION: Plays a role in tail tip complex assembly. The tail tip complex is assembled successively with three tail tip proteins J, one tail tip protein I, one tail tip protein L and one tail tip protein K. The tail tip complex interacts with tail measure protein to initiate tail tube assembly. The formation of the tail tip complex is completed by the addition of tail tip protein M, which is followed by tail tube polymerization. May be excluded form tail tip during maturation and would be absent from virions. May be involved in tail measure protein processing. {ECO:0000250|UniProtKB:P03729}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Domain (2); Metal binding (3); Motif (1)
Keywords Host cytoplasm;Hydrolase;Late protein;Metal-binding;Metalloprotease;Protease;Reference proteome;Thiol protease;Viral release from host cell;Viral tail assembly;Zinc
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250|UniProtKB:P03729}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif MOTIF 69..82; /note=JAMM motif; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182
Gene Encoded By
Mass 28,325
Kinetics
Metal Binding METAL 69; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 71; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182; METAL 82; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01182
Rhea ID
Cross Reference Brenda