| IED ID | IndEnz0002001406 |
| Enzyme Type ID | protease001406 |
| Protein Name |
Tape measure protein TMP Gene product H gpH Minor tail protein H Cleaved into: Entry protein H* |
| Gene Name | H lambdap16 |
| Organism | Escherichia phage lambda (Bacteriophage lambda) |
| Taxonomic Lineage | Viruses Duplodnaviria Heunggongvirae Uroviricota Caudoviricetes Caudovirales Siphoviridae (phages with long non-contractile tails) Lambdavirus Escherichia phage lambda (Bacteriophage lambda) |
| Enzyme Sequence | MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVVEQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKDSFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQAGLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGVEVDKVAEAFGKLTTDPTSGLTAMARQFHNVSAEQIAYVAQLQRSGDEAGALQAANEAATKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAYKKADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPKQSSVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQLRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR |
| Enzyme Length | 853 |
| Uniprot Accession Number | P03736 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Serves as a ruler that controls the length of tail by stopping the tail tube polymerization and is probably released from the tail shaft during infection to facilitate DNA translocation into the host cell (PubMed:2150582). Assembles into a multimeric linear form possibly stabilized by the covering tail assembly proteins G and GT (PubMed:23911548). Its C-terminus fixes the tail tip complex (J, I, L, K), thereby forming the tail assembly initiator complex (PubMed:23911548). Tail tube proteins polymerize around tape measure protein, displacing the tail assembly protein G and GT (PubMed:23911548). When the tail reaches the length specified by the tape measure protein, it stops and becomes capped by the tail terminator protein (Probable). Upon tail assembly, tape measure protein is cleaved into a form called H*, that plays a role later during virion entry in a new cell (Probable). Once assembled, the virion is released and can infect new cell by binding to the entry receptor LambB (Probable). After opening of a pore on the external membrane, the entry protein H* protein is probably released in the periplasmic space for successfull DNA injection (PubMed:23911548). {ECO:0000255|HAMAP-Rule:MF_04138, ECO:0000269|PubMed:2150582, ECO:0000269|PubMed:23911548, ECO:0000303|PubMed:23911548, ECO:0000305|PubMed:1108413, ECO:0000305|PubMed:23911548, ECO:0000305|PubMed:6220513, ECO:0000305|PubMed:6228546}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (2); Coiled coil (2); Region (1) |
| Keywords | Coiled coil;Reference proteome;Viral genome ejection through host cell envelope;Viral long flexible tail ejection system;Viral penetration into host cytoplasm;Viral release from host cell;Viral tail assembly;Viral tail protein;Virion;Virus entry into host cell |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04138, ECO:0000269|PubMed:4476800}. Note=There are 6 copies of entry protein H* per mature phage. {ECO:0000255|HAMAP-Rule:MF_04138, ECO:0000269|PubMed:4476800}. |
| Modified Residue | |
| Post Translational Modification | PTM: Cleaved into mature protein H* by an unidentified protease. {ECO:0000255|HAMAP-Rule:MF_04138, ECO:0000269|PubMed:6220513}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 21943085; |
| Motif | |
| Gene Encoded By | |
| Mass | 92,280 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |