| IED ID | IndEnz0002001417 |
| Enzyme Type ID | protease001417 |
| Protein Name |
Metalloprotease TIKI2 EC 3.4.-.- Heart, kidney and adipose-enriched transmembrane protein TRAB domain-containing protein 2B |
| Gene Name | Trabd2b Gm12824 Hkat Tiki2 |
| Organism | Mus musculus (Mouse) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Murinae Mus Mus Mus musculus (Mouse) |
| Enzyme Sequence | MHAALAGPLLAALLATARARPQPPDGGQCRPPGSQRDLNSFLWTIRRHPPAYLFGTIHVPYTRVWDFIPDNSKAAFQASTHVYFELDLTDPYTISALASCQLLPHGENLQDVLPRELYWRLKRHLDYVKLMIPSWMTPAQRGKGLYADYLFNAIAGNWERKRPVWVMLMVNSLTETDVRSRGVPVLDLYLAQQAEKMKKSTGAVERVEEQCHPLNGLNFSQVLFALNQTLLQHESVRAGSLQAPYTTEDLIKHYNCGDLNAVIFNHDTSQLPNFINTTLPPHEQVTAQEIDSYFRQELIYKRNERMGKRVMALLQENQDKICFFAFGAGHFLGNNTVIDVLRQAGLEVDHTPAGQAIHGPAAVGSPAPPPEITSPASPAPATPAAAVPEATSATPTTPPEEEDPVLSPHLLLPDSLSQLEEFGRQKWRKRLNKHQRPRQFNDLWVRIEDSTTISPPPLPLQPTPSSETTKPFVKSSHQLQQQDAVGPTSSSAPTLGLLHAITASIVAPFLLHSLGPS |
| Enzyme Length | 517 |
| Uniprot Accession Number | B1ATG9 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.-.- |
| Enzyme Function | FUNCTION: Metalloprotease that acts as a negative regulator of the Wnt signaling pathway by mediating the cleavage of the 8 N-terminal residues of a subset of Wnt proteins. Following cleavage, Wnt proteins become oxidized and form large disulfide-bond oligomers, leading to their inactivation. Able to cleave WNT3A, WNT5, but not WNT11. Required for head formation (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Compositional bias (2); Glycosylation (2); Region (2); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | Cell membrane;Glycoprotein;Hydrolase;Membrane;Metal-binding;Metalloprotease;Protease;Reference proteome;Signal;Transmembrane;Transmembrane helix;Wnt signaling pathway |
| Interact With | |
| Induction | INDUCTION: Transiently induced during 3T3-L1 cell differentiation into adipocytes. In vivo, down-regulated by fasting in both white and brown adipose tissues. High-fat diet down-regulates expression in white adipose tissue and up-regulates it in brown adipose tissue. {ECO:0000269|PubMed:23152936}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23152936}; Single-pass type I membrane protein {ECO:0000269|PubMed:23152936}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 25354456; 29428562; 31784461; |
| Motif | |
| Gene Encoded By | |
| Mass | 57,368 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |