| IED ID | IndEnz0002001429 |
| Enzyme Type ID | protease001429 |
| Protein Name |
26S proteasome regulatory subunit 4 homolog B 26S proteasome AAA-ATPase subunit RPT2b 26S proteasome subunit 4 homolog B Regulatory particle triple-A ATPase subunit 2b |
| Gene Name | RPT2B At2g20140 T2G17.6 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MGQGPSGGLNRQGDRKPDGGEKKEKKFEPAAPPARVGRKQRKQKGPEAAARLPTVTPSTKCKLRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEEDRSKVDDLRGTPMSVGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMKVEKAPLESYADIGGLEAQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLAKAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDANSGGEREIQRTMLELLNQLDGFDSRGDVKVILATNRIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFQIHTSKMTLAEDVNLEEFVMTKDEFSGADIKAICTEAGLLALRERRMKVTHVDFKKAKEKVMFKKKEGVPEGLYM |
| Enzyme Length | 443 |
| Uniprot Accession Number | Q9SL67 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (PubMed:22158466). Acts redundantly with RPT2A in the regulation of gametogenesis (PubMed:21784786, PubMed:22158466). With RPT2A plays a critical role in 26S proteasome assembly (PubMed:22158466). {ECO:0000269|PubMed:21784786, ECO:0000269|PubMed:22158466}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | NP_BIND 229..236; /note=ATP; /evidence=ECO:0000255 |
| Features | Chain (1); Compositional bias (2); Cross-link (2); Nucleotide binding (1); Region (2); Sequence conflict (3) |
| Keywords | ATP-binding;Cytoplasm;Isopeptide bond;Nucleotide-binding;Nucleus;Proteasome;Reference proteome;Ubl conjugation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22158466}. Nucleus {ECO:0000269|PubMed:22158466}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12668628; 12912986; 17317660; 17675552; 17825468; 20118269; 20706207; |
| Motif | |
| Gene Encoded By | |
| Mass | 49,347 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |