Detail Information for IndEnz0002001453
IED ID IndEnz0002001453
Enzyme Type ID protease001453
Protein Name Penicillin-binding protein 2A
PBP-2B
EC 3.4.16.4
Gene Name pbpA yqgF BSU25000
Organism Bacillus subtilis (strain 168)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168)
Enzyme Sequence MRRNKPKKQNHKEKKKSLPIRLNILFLAAFVIFTWIIVELGIKQIVQGDDYKNQANKQEQSEVSSAVPRGKIYDRNFNAIVTNKALNAITYTRSKSTTQEQRLKIAKKLSDMIKVDTKKVTERDKKDYWILTRPKEAKKLISSKERQQVEDKKISDDDLYQLQLKRITDKQLNELTDKDMQILAIKRQMDSGYALTPQYIKNEDVSAKEMAVVSEHLDELPGVDVTSDWEREYPYKNLLRSVLGSVSSSNEGLPSNLLDHYLSLGYSRNDRVGKSYLEYQYESLLQGQKAKVENITDSKGNVTGTKTVSEGKAGKDLVLTIDIDLQKSVEKIIEKKLKAAKARPSTELLDRAFVVMMDPRNGEVLTMAGKQIKRENGAYKFDDYALGAMTSSYAMGSAVKGATVLTGLQTGAINLNTVFKDEPLYIGQDKRGKKSWQNLGPVGIQTALEKSSNVFMFKTAIAVGKGEYKPHQALPLDTSAFDTFRNYFSQFGLGVKTGIDLPNEMTGYKGTSRLSGFLLDFAIGQYDTYTPLELAQYVSTIANGGYRMKPQLVKEVRDSNAKKGIGAVVDSVQPEVLNKVDMKSSYIEEVQAGFRRVATKGTAAGQLASASYKPAAKTGTAQSFYDGPDKSKTGTDTYNTTLVAYAPADNPEIAISVVVPWTYIDYNQRYSITNEIGREVMDKYFELKSKQDKEGTQQKNKDKIEENAENTTSSDN
Enzyme Length 716
Uniprot Accession Number P54488
Absorption
Active Site ACT_SITE 397; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:P0AD65
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.; EC=3.4.16.4; Evidence={ECO:0000305};
DNA Binding
EC Number 3.4.16.4
Enzyme Function FUNCTION: Involved in the synthesis of peptidoglycan associated with cell wall elongation, especially following spore germination (PubMed:9139922). Has a partially redundant function with PBP 1 (ponA) or PBP 4 (pbpD) during spore outgrowth (PubMed:9851991). Plays a redundant role with PbpH in determining the rod shape of the cell during vegetative growth and spore outgrowth (PubMed:12896990). {ECO:0000269|PubMed:12896990, ECO:0000269|PubMed:9139922, ECO:0000269|PubMed:9851991}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
nucleotide Binding
Features Active site (1); Chain (1); Compositional bias (1); Region (1); Transmembrane (1)
Keywords Antibiotic resistance;Carboxypeptidase;Cell membrane;Cell shape;Cell wall biogenesis/degradation;Direct protein sequencing;Hydrolase;Membrane;Peptidoglycan synthesis;Protease;Reference proteome;Transmembrane;Transmembrane helix
Interact With
Induction INDUCTION: Transcribed during vegetative phase, drops off during sporulation, rises again 30-40 minutes after germination. A small amount is present in dormant spores. {ECO:0000269|PubMed:9139922}.
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:3080407}. Forespore inner membrane {ECO:0000269|PubMed:3080407}; Single-pass membrane protein {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 80,142
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda