| IED ID | IndEnz0002001469 |
| Enzyme Type ID | protease001469 |
| Protein Name |
Plasmepsin V PvPMV EC 3.4.23.- Plasmepsin 5 |
| Gene Name | PMV PVX_116695 |
| Organism | Plasmodium vivax (strain Salvador I) |
| Taxonomic Lineage | cellular organisms Eukaryota Sar Alveolata Apicomplexa Aconoidasida Haemosporida (haemosporidians) Plasmodiidae Plasmodium Plasmodium (Plasmodium) Plasmodium vivax Plasmodium vivax (strain Salvador I) |
| Enzyme Sequence | MVGASLGPPGRGSLSRLIRLVICVLTLCALSVQGRSESTEGHSKDLLYKYKLYGDIDEYAYYFLDIDIGTPEQRISLILDTGSSSLSFPCAGCKNCGVHMENPFNLNNSKTSSILYCENEECPFKLNCVKGKCEYMQSYCEGSQISGFYFSDVVSVVSYNNERVTFRKLMGCHMHEESLFLYQQATGVLGMSLSKPQGIPTFVNLLFDNAPQLKQVFTICISENGGELIAGGYDPAYIVRRGGSKSVSGQGSGPVSESLSESGEDPQVALREAEKVVWENVTRKYYYYIKVRGLDMFGTNMMSSSKGLEMLVDSGSTFTHIPEDLYNKLNYFFDILCIQDMNNAYDVNKRLKMTNESFNNPLVQFDDFRKSLKSIIAKENMCVKIVDGVQCWKYLEGLPDLFVTLSNNYKMKWQPHSYLYKKESFWCKGIEKQVNNKPILGLTFFKNRQVIFDIQKNRIGFVDANCPSHPTHTRPRTYNEYKRKDNIFLKIPFFYLYSLFVVFALSVLLSLVFYVRRLYHMEYSPLPSEGKAPADA |
| Enzyme Length | 536 |
| Uniprot Accession Number | A5K302 |
| Absorption | |
| Active Site | ACT_SITE 80; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103; ACT_SITE 313; /evidence=ECO:0000255|PROSITE-ProRule:PRU01103 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by peptidomimetic inhibitors such as WEHI-842. {ECO:0000269|PubMed:26214367, ECO:0000269|PubMed:29800827}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.23.- |
| Enzyme Function | FUNCTION: During the asexual blood stage, plays an essential role in the export of several proteins into the host erythrocytes by cleaving the pentameric localization motif RxLxE/Q/D (termed Plasmodium export element (PEXEL)) located downstream of the N-terminal secretory signal sequence (PubMed:24983235). Specifically, cleaves after the leucine residue in the RxLxE/Q/D (or RxLxxE) motif of exported proteins including EMP1 (By similarity). Also, by regulating protein export, plays an essential role in gametocyte development and thus parasite transmission to the mosquito vector (By similarity). {ECO:0000250|UniProtKB:Q8I6Z5, ECO:0000250|UniProtKB:W7JPD9, ECO:0000269|PubMed:24983235}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Disulfide bond (7); Domain (1); Mutagenesis (2); Region (1); Signal peptide (1); Topological domain (2); Transmembrane (1) |
| Keywords | 3D-structure;Aspartyl protease;Disulfide bond;Endoplasmic reticulum;Hydrolase;Membrane;Protease;Reference proteome;Signal;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:24983235}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q8I6Z5}. |
| Modified Residue | |
| Post Translational Modification | PTM: It is not clear if the zymogen has a cleavable propeptide (By similarity). Cleavage of the putative propeptide is dispensable for catalytic activity (By similarity). {ECO:0000250|UniProtKB:Q8I6Z5}. |
| Signal Peptide | SIGNAL 1..34; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 4ZL4; 6C4G; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 60,916 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |