| IED ID | IndEnz0002001489 |
| Enzyme Type ID | protease001489 |
| Protein Name |
Polyprotein pp62 60 kDa polyprotein p60 62 kDa polyprotein p62 Cleaved into: p15; p35 PIG1 UB18 ; p8 |
| Gene Name | Ba71V-94 CP530R |
| Organism | African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV) |
| Taxonomic Lineage | Viruses Varidnaviria Bamfordvirae Nucleocytoviricota Pokkesviricetes Asfuvirales Asfarviridae Asfivirus African swine fever virus (ASFV) African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV) |
| Enzyme Sequence | MPSNMKQFCKISVWLQQHDPDLLEIINNLCMLGNLSAAKYKHGVTFIYPKQAKIRDEIKKHAYSNDPSQAIKTLESLILPFYIPTPAEFTGEIGSYTGVKLEVEKTEANKVILKNGEAVLVPAADFKPFPDRRLAVWIMESGSMPLEGPPYKRKKEGGGNDPPVPKHISPYTPRTRIAIEVEKAFDDCMRQNWCSVNNPYLAKSVSLLSFLSLNHPTEFIKVLPLIDFDPLVTFYLLLEPYKTHGDDFLIPETILFGPTGWNGTDLYQSAMLEFKKFFTQITRQTFMDIADSATKEVDVPICYSDPETVHSYTNHVRTEILHHNAVNKVTTPNLVVQAYNELEQTNTIRHYGPIFPESTINALRFWKKLWQDEQRFVIHGLHRTLMDQPTYETSEFAEIVRNLRFSRPGNNYINELNITSPAMYGDKHTTGDIAPNDRFAMLVAFINSTDFLYTAIPEEKVGGNETQTSSLTDLVPTRLHSFLNHNLSKLKILNRAQQTVRNILSNDCLNQLKHYVKHTGKNEILKLLQE |
| Enzyme Length | 530 |
| Uniprot Accession Number | Q65179 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: [Polyprotein pp62]: Essential for the correct assembly and maturation of the core of the virion. {ECO:0000269|PubMed:19846532}.; FUNCTION: [p35]: Component of the core shell (PubMed:30185597). Binds to phosphatidylserine, which may enable the core shell binding with the inner membrane (PubMed:32519301). {ECO:0000269|PubMed:30185597, ECO:0000269|PubMed:32519301}.; FUNCTION: [p15]: Component of the core shell (PubMed:30185597). Binds to phosphatidylserine and DNA, which may link the core shell to the inner membrane and to the viral nucleoid (PubMed:32451720). {ECO:0000269|PubMed:30185597, ECO:0000269|PubMed:32451720}.; FUNCTION: [p8]: Component of the core shell. {ECO:0000269|PubMed:30185597}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (11); Chain (4); Disulfide bond (2); Frameshift (1); Helix (23); Initiator methionine (1); Mutagenesis (2); Natural variant (8); Site (4); Turn (4) |
| Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Host cytoplasm;Late protein;Reference proteome;Ubl conjugation;Virion |
| Interact With | |
| Induction | INDUCTION: Expressed in the late phase of the viral replicative cycle. {ECO:0000269|PubMed:32075923}. |
| Subcellular Location | SUBCELLULAR LOCATION: [Polyprotein pp62]: Host cytoplasm, host perinuclear region {ECO:0000269|PubMed:9223468}. Note=Found in perinuclear cytoplasmic viral factories during assembly. {ECO:0000269|PubMed:9223468}.; SUBCELLULAR LOCATION: [p35]: Virion {ECO:0000269|PubMed:30185597, ECO:0000269|PubMed:33629764}. Note=Located in the core shell, which functions like a matrix between the DNA and the inner envelope. {ECO:0000269|PubMed:33629764}.; SUBCELLULAR LOCATION: [p15]: Virion {ECO:0000269|PubMed:30185597, ECO:0000269|PubMed:33629764}. Note=Located in the core shell, which functions like a matrix between the DNA and the inner envelope. {ECO:0000269|PubMed:33629764}.; SUBCELLULAR LOCATION: [p8]: Virion {ECO:0000269|PubMed:30185597}. Note=Located in the core shell, which functions like a matrix between the DNA and the inner envelope. {ECO:0000303|PubMed:30185597}. |
| Modified Residue | |
| Post Translational Modification | PTM: [p15]: Monoubiquitinated in vitro by viral UBCv1. {ECO:0000269|PubMed:7853518}.; PTM: [Polyprotein pp62]: Specific enzymatic cleavages in vivo by the viral pS273R protease yield mature proteins. {ECO:0000269|PubMed:30185597, ECO:0000269|PubMed:9223468}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 6NLN; 7BQ9; 7BQA; 7CP2; |
| Mapped Pubmed ID | 31038945; |
| Motif | |
| Gene Encoded By | |
| Mass | 60,547 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |