| IED ID | IndEnz0002001491 |
| Enzyme Type ID | protease001491 |
| Protein Name |
26S proteasome non-ATPase regulatory subunit 8 homolog A 26S proteasome regulatory subunit RPN12a AtRPN12a 26S proteasome regulatory subunit S14 homolog A |
| Gene Name | RPN12A At1g64520 F1N19.9 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MDPQLTEVSQQFERFKAAFARKDYNTCSDLLSQLKVLLTKFTSLPPLFENSPNAAKELTIARDIYEHAVVLSVKTEDQDAFERDFFQLKPYYVDARNRIPQSPQENLILGLNLLRLLVQNRIAEFHTELELLSSATLEDPCIKHAVELEQSFMEGAYNRVLSARQTAPDATYVYFMDLLAKTIRDEIAGCSEKAYDYVSISDARQMLLFSSDQELLTYVTDEHPEWEVKEGFVVFQKAKETAPCKEIPSLQLINQTLSYARELERIV |
| Enzyme Length | 267 |
| Uniprot Accession Number | Q9SGW3 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. May help to control the degradation of one or more factors that repress cytokinin signaling. Plays an important role for balancing cell expansion with cell proliferation rates during shoot development. {ECO:0000269|PubMed:11826296, ECO:0000269|PubMed:17971041, ECO:0000269|PubMed:19321709, ECO:0000269|PubMed:19812900}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Modified residue (1); Sequence conflict (1) |
| Keywords | Acetylation;Cytokinin signaling pathway;Proteasome;Reference proteome;Ubl conjugation |
| Interact With | P46639; Q9LQF0 |
| Induction | INDUCTION: By cytokinins. {ECO:0000269|PubMed:11826296}. |
| Subcellular Location | |
| Modified Residue | MOD_RES 1; /note="N-acetylmethionine"; /evidence="ECO:0000269|PubMed:20516081, ECO:0007744|PubMed:22223895" |
| Post Translational Modification | PTM: Ubiquitinated by PUB22 and PUB23. {ECO:0000269|PubMed:18664614}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10363660; 11742986; 12766230; 12969430; 14749482; 17426018; 17825468; 18650403; 20087596; 20118269; 20706207; 21798944; 22323770; 27247031; 27613851; 28491076; 28627464; |
| Motif | |
| Gene Encoded By | |
| Mass | 30,706 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |