| IED ID | IndEnz0002001515 |
| Enzyme Type ID | protease001515 |
| Protein Name |
Tripeptidyl-peptidase 2 EC 3.4.14.10 Tripeptidyl-peptidase II TPPII |
| Gene Name | TPP2 At4g20850 T13K14.10 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MDLSLQLQIHGALINKGPSCTSYWASSSSLSLPRDFISSSTFLLHRRLRRRSCSRSRGIRLRRSGFSAMPCSSSDTLTASRVGCGGGGGGGAVGGGAENASVANFKLNESTFIASLMPKKEIRADCFIEAHPEYDGRGVVIAIFDSGFDPSAAGLHVTSDGKPKVLDVIDCTGSGDIDTSTVVKANEDGHIRGASGATLVVNSSWKNPTGEWRVGSKLVYQLFTDDLTSRVKKERRKSWDEKNQEEIAKAVNNLYDFDQKHSKVEDAKLKKTREDLQSKVDFLKKQADKYEDKGPVIDAVVWHDGEVWRVALDTQSLEEDPDSGKLADFSPLTNYRIERKYGVFSRLDACSFVANVYDEGKVLSIVTDSSPHGTHVAGIATAHHPEEHLLNGVAPGAQIISCKIGDSRLGSMETGTGLTRALIAALEHNCDLVNMSYGEPALLPDYGRFVDLVTEAVNKRRLIFVSSAGNSGPALTTVGAPGGTTSSIIGVGAYVSPAMAAGAHSVVEPPSEGLEYTWSSRGPTSDGDLGVCISAPGGAVAPVPTWTLQRRMLMNGTSMASPSACGAIALLLSAMKAEGIPVSPYSVRRALENTSTPVGDLPEDKLTTGQGLMQVDKAYEYLKQFQDYPCVFYQIKVNLSGKTIPTSRGIYLREGTACRQSTEWTIQVDPKFHEGASNLKELVPFEECLELHSTDEGVVRVPDYLLLTNNGRGFNVVVDPTNLGDGVHYFEVYGIDCKAPERGPLFRIPVTIIIPKTVANQPPVISFQQMSFISGHIERRYIEVPHGATWAEATMRTSGFDTTRRFYIDTLQVCPLRRPIKWESAPTFASPSAKSFVFPVVSGQTMELAIAQFWSSGLGSREPTIVDFEIEFHGVGVDKEELLLDGSEAPIKVEAEALLASEKLVPIAVLNKIRVPYQPIDAQLKTLSTGRDRLLSGKQILALTLTYKFKLEDSAEVKPYIPLLNNRIYDTKFESQFFMISDTNKRVYAMGDVYPESSKLPKGEYKLQLYLRHENVELLEKLKQLTVFIERNMGEIRLNLHSEPDGPFTGNGAFKSSVLMPGVKEAFYLGPPTKDKLPKNTPQGSMLVGEISYGKLSFDEKEGKNPKDNPVSYPISYVVPPNKPEEDKKAASAPTCSKSVSERLEQEVRDTKIKFLGNLKQETEEERSEWRKLCTCLKSEYPDYTPLLAKILEGLLSRSDAGDKISHHEEIIEAANEVVRSVDVDELARFLLDKTEPEDDEAEKLKKKMEVTRDQLADALYQKGLAMARIENLKGEKEGEGEEESSQKDKFEENFKELTKWVDVKSSKYGTLTVLREKRLSRLGTALKVLDDLIQNENETANKKLYELKLDLLEEIGWSHLVTYEKQWMQVRFPKSLPLF |
| Enzyme Length | 1380 |
| Uniprot Accession Number | F4JVN6 |
| Absorption | |
| Active Site | ACT_SITE 145; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 372; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 558; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by alanine-alanine-phenylalanine-chloromethylketone, butabindide and phenylmethanesulfonyl fluoride (PMSF), but not by leupeptin, N-ethylmaleimide, EDTA, MG132 and lactacystin. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of an N-terminal tripeptide from a polypeptide.; EC=3.4.14.10; Evidence={ECO:0000269|PubMed:15908606, ECO:0000269|PubMed:19822524}; |
| DNA Binding | |
| EC Number | 3.4.14.10 |
| Enzyme Function | FUNCTION: Serine protease of the proteasome pathway that may function with the 20S proteasome to degrade oxidized proteins generated by environmental stress. {ECO:0000269|PubMed:15908606, ECO:0000269|PubMed:19822524}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Coiled coil (2); Domain (1); Erroneous gene model prediction (2); Region (1) |
| Keywords | Aminopeptidase;Coiled coil;Hydrolase;Protease;Reference proteome;Serine protease;Stress response |
| Interact With | |
| Induction | INDUCTION: By cadmium (at protein level). {ECO:0000269|PubMed:19822524}. |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14576160; 15028209; 15215502; 15539469; 15821981; 15998247; 16193095; 16679420; 16895613; 16972165; 17151019; 17432890; 18431481; 18775970; 19136719; 21166475; 21764588; 28627464; 32344669; |
| Motif | |
| Gene Encoded By | |
| Mass | 152,368 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |