| IED ID | IndEnz0002001528 |
| Enzyme Type ID | protease001528 |
| Protein Name |
Sortase A EC 3.4.22.- |
| Gene Name | strA gbs0949 |
| Organism | Streptococcus agalactiae serotype III (strain NEM316) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Lactobacillales Streptococcaceae Streptococcus Streptococcus agalactiae Streptococcus agalactiae serogroup III Streptococcus agalactiae serotype III (strain NEM316) |
| Enzyme Sequence | MRNKKKLHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQSNHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVEINLPIFKGLGNTELSYGAGTMKENQIMGGQNNYALASHHVFGLTGSSKMLFSPLEHAKKGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVHAELEKTGEFSTADESILKAFSKKYNQINL |
| Enzyme Length | 247 |
| Uniprot Accession Number | Q8E5N2 |
| Absorption | |
| Active Site | ACT_SITE 140; /note=Proton donor/acceptor; /evidence=ECO:0000250|UniProtKB:Q2FV99; ACT_SITE 206; /note=Acyl-thioester intermediate; /evidence=ECO:0000250|UniProtKB:Q2FV99 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Transpeptidase that anchors surface proteins to the cell wall. Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall. This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues (By similarity). Essential for adherence to eukaryotic cells and for binding to fibronectin and fibrinogen (PubMed:15908360). {ECO:0000250|UniProtKB:Q2FV99, ECO:0000269|PubMed:15908360}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Site (1); Topological domain (2); Transmembrane (1) |
| Keywords | Cell membrane;Hydrolase;Membrane;Protease;Thiol protease;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q2FV99}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:Q2FV99}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 27,504 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |