| IED ID | IndEnz0002001643 |
| Enzyme Type ID | protease001643 |
| Protein Name |
RNA polymerase sigma factor RpoH Heat shock regulatory protein F33.4 RNA polymerase sigma-32 factor |
| Gene Name | rpoH fam hin htpR b3461 JW3426 |
| Organism | Escherichia coli (strain K12) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Escherichia Escherichia coli Escherichia coli (strain K12) |
| Enzyme Sequence | MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYHGDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARELGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA |
| Enzyme Length | 284 |
| Uniprot Accession Number | P0AGB3 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | DNA_BIND 253..272; /note=H-T-H motif; /evidence=ECO:0000255|HAMAP-Rule:MF_00961 |
| EC Number | |
| Enzyme Function | FUNCTION: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes. Intracellular concentration of free RpoH protein increases in response to heat shock, which causes association with RNA polymerase (RNAP) and initiation of transcription of heat shock genes, including numerous global transcriptional regulators and genes involved in maintaining membrane functionality and homeostasis. RpoH is then quickly degraded, leading to a decrease in the rate of synthesis of heat shock proteins and shut-off of the heat shock response. {ECO:0000255|HAMAP-Rule:MF_00961, ECO:0000269|PubMed:15757896, ECO:0000269|PubMed:16818608, ECO:0000269|PubMed:3306410, ECO:0000269|PubMed:3315848, ECO:0000269|PubMed:6387714}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); DNA binding (1); Motif (1); Mutagenesis (1); Region (2); Sequence conflict (2) |
| Keywords | Cytoplasm;DNA-binding;Reference proteome;Sigma factor;Stress response;Transcription;Transcription regulation |
| Interact With | P0A6Y8; P0AGD7; P0AAI3; P0A6F5 |
| Induction | INDUCTION: Subject to complex regulation at multiple levels (transcription, translation, regulation of activity and degradation). In the absence of heat shock, or after heat shock, activity is inhibited by transient association with DnaK and DnaJ, which reduces the amounts of free active RpoH, makes it unstable and mediates its degradation by the FtsH protease. During heat shock, the intracellular concentration of RpoH increases, due to slightly increased transcription, increased synthesis and stabilization of the protein. Induction occurs mainly at the post-transcriptional level, via translational thermoregulation: at low temperature, the structure of the rpoH mRNA blocks its translation, while at high temperature, melting of the mRNA secondary structure facilitates ribosome binding and synthesis of the RpoH protein. In addition, during heat shock, stabilization of RpoH is triggered by the titration of free DnaK/DnaJ by stress-induced misfolded proteins. Can also be induced by other stress conditions, including during the first round of cell division. {ECO:0000269|PubMed:10090722, ECO:0000269|PubMed:15757896, ECO:0000269|PubMed:19114495, ECO:0000269|PubMed:3306410, ECO:0000269|PubMed:3315848, ECO:0000269|PubMed:3315851, ECO:0000269|PubMed:8599944, ECO:0000269|PubMed:9822823, ECO:0000269|PubMed:9882652}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00961}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 15690043; 16606699; 26545493; 6337122; |
| Motif | MOTIF 77..80; /note=Interaction with polymerase core subunit RpoC |
| Gene Encoded By | |
| Mass | 32,469 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |