| IED ID | IndEnz0002001649 |
| Enzyme Type ID | protease001649 |
| Protein Name |
Deubiquitinase SseL EC 3.4.22.- Deubiquitinating enzyme DUB Deubiquitinating protease Salmonella secreted effector L |
| Gene Name | sseL elaD SCH_2290 |
| Organism | Salmonella choleraesuis (strain SC-B67) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella enterica subsp. enterica serovar Choleraesuis Salmonella choleraesuis (strain SC-B67) |
| Enzyme Sequence | MNICVNSLYRLSTPQFHSLYSEDVSDEVLALLIGEVENGNQNCIDLLCNLALRNDDLGHKVEKLLFDLFSGKRSGSPDIDKKINQACLVLHQIANNDITKNNTEWKKLHAPSRLLYMAGSATTDLSKKIGIAHKIMGDQFAQTDQEQVGVENLWCSARMLSSDELAAATQGLVQESPFLSVNYPIGLIHPTTKENILSTQLLEKMAQSGLSENEVFLINTGDHWLICLFYKLAEKIKCLIFNTYYDLNENTKQEIIEAAKIAGISESDEVNFIEMNLQNNVPNGCSLFCYHTIQLLSNAGQNDPVTTLREFAEKFLTLSVEEQALFNTQTRRQIYEYSLQ |
| Enzyme Length | 340 |
| Uniprot Accession Number | Q57M66 |
| Absorption | |
| Active Site | ACT_SITE 223; /evidence=ECO:0000250; ACT_SITE 285; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protease targets the host cell ubiquitin pathway by acting as a deubiquitinase in infected host cells (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1) |
| Keywords | Host cytoplasm;Hydrolase;Protease;Secreted;Thiol protease;Virulence |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Host cytoplasm {ECO:0000250}. Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and delivered into the host cytoplasm. In phagocytic cells localizes to the Salmonella-containing vacuole (SCV). In epithelial cells localizes to the Salmonella-containing vacuole (SCV) and to the Salmonella-induced filaments (Sifs), which are tubular membrane extensions from the SCV that are formed at late stages of infection (By similarity). {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 38,265 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |