| IED ID | IndEnz0002001655 |
| Enzyme Type ID | protease001655 |
| Protein Name |
Ribosomal RNA small subunit methyltransferase J EC 2.1.1.242 16S rRNA m2G1516 methyltransferase rRNA guanine-N 2 - -methyltransferase |
| Gene Name | rsmJ yhiQ STM3593 |
| Organism | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Enterobacteriaceae Salmonella Salmonella enterica (Salmonella choleraesuis) Salmonella enterica I Salmonella typhimurium Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
| Enzyme Sequence | MQICLMDETGATDGALSVLAARWGLEHDEDNPMALVMTPQHLELRKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRDAFVLASVGCRVRMLERNPVVAALLDDGLTRGYADADIGGWLQERLQLIHASSLTALTDITPRPQVVYLDPMFPHRQKSALVKKEMRVFQSLVGPDLDADGLLEPARQLATKRVVVKRPDYAPPLADVATPNAIVTKGHRFDIYAGTPLTE |
| Enzyme Length | 252 |
| Uniprot Accession Number | Q9X6G2 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 171; /note=S-adenosyl-L-methionine; /evidence=ECO:0000255|HAMAP-Rule:MF_01523 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=guanosine(1516) in 16S rRNA + S-adenosyl-L-methionine = H(+) + N(2)-methylguanosine(1516) in 16S rRNA + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:43220, Rhea:RHEA-COMP:10412, Rhea:RHEA-COMP:10413, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.242; Evidence={ECO:0000255|HAMAP-Rule:MF_01523}; |
| DNA Binding | |
| EC Number | 2.1.1.242 |
| Enzyme Function | FUNCTION: Specifically methylates the guanosine in position 1516 of 16S rRNA. {ECO:0000255|HAMAP-Rule:MF_01523}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Beta strand (12); Binding site (1); Chain (1); Helix (12); Region (3); Sequence conflict (1); Turn (2) |
| Keywords | 3D-structure;Cytoplasm;Methyltransferase;Reference proteome;S-adenosyl-L-methionine;Transferase;rRNA processing |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01523}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 2PKW; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 27,300 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:43220 |
| Cross Reference Brenda |