IED Industry Enzyme Database

Detail Information for IndEnz0002001688
IED ID IndEnz0002001688
Enzyme Type ID protease001688
Protein Name Metalloproteinase inhibitor 2
CSC-21K
Tissue inhibitor of metalloproteinases 2
TIMP-2
Gene Name TIMP2
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MGAAARTLRLALGLLLLATLLRPADACSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPEKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSTTQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP
Enzyme Length 220
Uniprot Accession Number P16035
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. {ECO:0000269|PubMed:11710594, ECO:0000269|PubMed:2554304, ECO:0000269|PubMed:2793861}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (14); Chain (1); Disulfide bond (6); Domain (1); Helix (7); Metal binding (1); Region (2); Sequence conflict (17); Signal peptide (1); Site (3); Turn (2)
Keywords 3D-structure;Direct protein sequencing;Disulfide bond;Metal-binding;Metalloenzyme inhibitor;Metalloprotease inhibitor;Protease inhibitor;Reference proteome;Secreted;Signal;Zinc
Interact With P50281; P08253; Q9UHD9
Induction INDUCTION: Down-regulated by TGFB1. {ECO:0000269|PubMed:2380196}.
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification PTM: The activity of TIMP2 is dependent on the presence of disulfide bonds.
Signal Peptide SIGNAL 1..26; /evidence="ECO:0000269|PubMed:1480041, ECO:0000269|PubMed:1730286, ECO:0000269|PubMed:2793861"
Structure 3D NMR spectroscopy (1); X-ray crystallography (3)
Cross Reference PDB 1BR9; 1GXD; 2TMP; 4ILW;
Mapped Pubmed ID 11606052; 11757622; 11912288; 12147251; 12372458; 12374789; 12406369; 12439941; 12446683; 12470034; 12475252; 12479097; 12602913; 12614934; 12887919; 12900406; 12970394; 12970724; 14517716; 14598888; 14604886; 14605322; 14654538; 14661256; 14710472; 14712492; 14744773; 14962256; 14973177; 15052627; 15056834; 15147743; 15193960; 15247230; 15248826; 15277439; 15308656; 15351863; 15489233; 15557756; 15567754; 15616792; 15628723; 15672858; 15691234; 15691353; 15754326; 15797648; 15841325; 15870703; 15944607; 16036783; 16042227; 16103240; 16142392; 16142692; 16237750; 16326706; 16424893; 16425263; 16429190; 16491114; 16623785; 16631927; 16716258; 16723886; 16728425; 16772717; 16776850; 16940985; 17008230; 17030988; 17067460; 17071711; 17106248; 17130843; 17203468; 17222415; 17226791; 17236757; 17325663; 17342343; 17350093; 17353931; 17374529; 17448043; 17493172; 17493602; 17495113; 17564313; 17572184; 17589947; 17613170; 17706812; 17786346; 17868665; 17886098; 17893005; 17901377; 17954022; 17979129; 17991754; 18025061; 18042068; 18177649; 18217401; 18265895; 18288718; 18291374; 18301898; 18329693; 18424416; 18469019; 18472160; 18474097; 18477480; 18487063; 18498066; 18543216; 18634035; 18674955; 18702016; 19020757; 19088110; 19141395; 19187648; 19241124; 19267203; 19317417; 19358835; 19383344; 19391484; 19431211; 19438747; 19464975; 19546837; 19551542; 19551841; 19596921; 19640333; 19698156; 19709349; 19723139; 19727008; 19758569; 19821096; 19844095; 19875168; 19883732; 19889076; 19913121; 19933216; 20041335; 20084057; 20149706; 20195229; 20215736; 20216978; 20233321; 20452482; 20484597; 20485444; 20514432; 20587546; 20598922; 20616161; 20627004; 20628086; 20634891; 20672350; 20673868; 20685029; 20694560; 20707923; 20718757; 20730428; 20889295; 20940305; 20941509; 20948207; 21086628; 21195432; 21228746; 21254651; 21261731; 21421877; 21427648; 21432777; 21437624; 21454617; 21462296; 21463121; 21478099; 21526499; 21620625; 21715326; 21786179; 21839835; 21871510; 22020421; 22051851; 22110735; 22122951; 22169692; 22200256; 22200690; 22270451; 22272343; 22302011; 22374248; 22427646; 22438955; 22511598; 22512703; 22532131; 22546345; 22551568; 22621753; 22685266; 2269296; 22730240; 22820140; 22880464; 22883459; 22985025; 22985578; 23038618; 23195431; 23205572; 23264224; 23320481; 23371049; 23388475; 23388612; 23412981; 23415672; 23427184; 23472970; 23491141; 23536957; 23563628; 23585206; 23650620; 23650722; 23661104; 23673111; 23708302; 23769926; 23791966; 23793312; 23817219; 23855590; 23973519; 24042342; 24068394; 24112707; 24202705; 24212294; 24335752; 24355694; 24384769; 24395652; 24403492; 24427326; 24440352; 24444603; 24475101; 24479343; 24534323; 24591757; 24675717; 24680659; 24710006; 24799419; 24800696; 24819588; 25060386; 25128867; 25136829; 25153995; 25189251; 25259564; 25300296; 25405519; 25412957; 25436070; 25524453; 25539555; 25552509; 25571919; 25579181; 25656607; 25731730; 25771900; 25826490; 25839957; 26067137; 26071077; 26130791; 26148591; 26191221; 26268417; 26278569; 26329758; 26331622; 26539028; 26551785; 26556867; 26765397; 26797672; 26823777; 26856768; 26978018; 26979530; 27018975; 27033700; 27058373; 27098106; 27142596; 27160811; 27173256; 27174659; 27174941; 27191265; 27329669; 27342580; 27342582; 27401679; 27412873; 27633774; 27834627; 27853937; 27901480; 28003188; 28035415; 28107490; 28137415; 28139355; 28192449; 28375112; 28382566; 28384717; 28498424; 28510611; 28620234; 28682920; 28731196; 28803769; 28871006; 28888475; 28980922; 29035450; 29103203; 29145491; 29265076; 29325453; 29385606; 29393341; 29432996; 29505725; 29658596; 29673878; 29685765; 29753168; 29763380; 29912573; 29930267; 29941993; 30227959; 30249138; 30272338; 30300282; 30515695; 30562560; 30583769; 30593723; 30676640; 30755371; 30765222; 30794673; 30982674; 30988064; 31018823; 31032338; 31061410; 31088428; 31119800; 31208828; 31263835; 31273966; 31298305; 31383784; 31545237; 31772230; 31791043; 31836008; 31856559; 31870265; 31887330; 31970775; 32116235; 32296833; 32479185; 32495877; 32542727; 32623572; 32661216; 32767336; 32945513; 32955903; 32961536; 32969251; 33009198; 33023532; 33045111; 33093251; 33270569; 33441876; 33471544; 33482846; 33725949; 34155539; 34487732; 34678879; 34732411; 7890645; 8314771; 8530478; 9422744; 9632662;
Motif
Gene Encoded By
Mass 24,399
Kinetics
Metal Binding METAL 27; /note="Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partner"; /evidence="ECO:0000269|PubMed:24073280, ECO:0007744|PDB:4ILW"
Rhea ID
Cross Reference Brenda 3.4.24.22;