| IED ID | IndEnz0002001717 |
| Enzyme Type ID | protease001717 |
| Protein Name |
E3 ubiquitin-protein ligase NRDP1 EC 2.3.2.27 RING finger protein 41 RING-type E3 ubiquitin transferase NRDP1 |
| Gene Name | RNF41 FLRF NRDP1 SBBI03 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MGYDVTRFQGDVDEDLICPICSGVLEEPVQAPHCEHAFCNACITQWFSQQQTCPVDRSVVTVAHLRPVPRIMRNMLSKLQIACDNAVFGCSAVVRLDNLMSHLSDCEHNPKRPVTCEQGCGLEMPKDELPNHNCIKHLRSVVQQQQTRIAELEKTSAEHKHQLAEQKRDIQLLKAYMRAIRSVNPNLQNLEETIEYNEILEWVNSLQPARVTRWGGMISTPDAVLQAVIKRSLVESGCPASIVNELIENAHERSWPQGLATLETRQMNRRYYENYVAKRIPGKQAVVVMACENQHMGDDMVQEPGLVMIFAHGVEEI |
| Enzyme Length | 317 |
| Uniprot Accession Number | Q9H4P4 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; |
| DNA Binding | |
| EC Number | 2.3.2.27 |
| Enzyme Function | FUNCTION: Acts as E3 ubiquitin-protein ligase and regulates the degradation of target proteins. Polyubiquitinates MYD88. Negatively regulates MYD88-dependent production of proinflammatory cytokines. Can promote TRIF-dependent production of type I interferon and inhibits infection with vesicular stomatitis virus (By similarity). Promotes also activation of TBK1 and IRF3. Involved in the ubiquitination of erythropoietin (EPO) and interleukin-3 (IL-3) receptors. Thus, through maintaining basal levels of cytokine receptors, RNF41 is involved in the control of hematopoietic progenitor cell differentiation into myeloerythroid lineages (By similarity). Contributes to the maintenance of steady-state ERBB3 levels by mediating its growth factor-independent degradation. Involved in the degradation of the inhibitor of apoptosis BIRC6 and thus is an important regulator of cell death by promoting apoptosis. Acts also as a PRKN modifier that accelerates its degradation, resulting in a reduction of PRKN activity, influencing the balance of intracellular redox state. The RNF41-PRKN pathway regulates autophagosome-lysosome fusion during late mitophagy. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control (PubMed:24949970). {ECO:0000250, ECO:0000250|UniProtKB:Q8BH75, ECO:0000269|PubMed:12411582, ECO:0000269|PubMed:14765125, ECO:0000269|PubMed:15632191, ECO:0000269|PubMed:17210635, ECO:0000269|PubMed:18541373, ECO:0000269|PubMed:19483718, ECO:0000269|PubMed:24949970}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Protein modification; protein ubiquitination. |
| nucleotide Binding | |
| Features | Alternative sequence (1); Beta strand (6); Chain (1); Erroneous initiation (1); Helix (8); Mutagenesis (3); Turn (3); Zinc finger (2) |
| Keywords | 3D-structure;Alternative splicing;Apoptosis;Autophagy;Metal-binding;Reference proteome;Transferase;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger |
| Interact With | A0A0C4DG62; Q66PJ3-3; Q9NWX5; Q9NX04; Q9UHY7; Q9NSB8; Q86U28; Q9BRK4; Q6FHY5; Q8N3F0; O15049; P54725; P35249; Itself; Q7LG56; Q9GZT4; Q8N8B7-2; Q99614; P61086; Q8N1B4 |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | PTM: Autoubiquitinated. Autoubiquitination leads to proteasomal degradation. Deubiquitinated by USP8 to get stabilized which induces apoptosis. {ECO:0000269|PubMed:15314180, ECO:0000269|PubMed:17210635, ECO:0000269|PubMed:19483718}. |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (2) |
| Cross Reference PDB | 2FZP; 2GWF; |
| Mapped Pubmed ID | 11358394; 11867753; 12646216; 14676191; 16954532; 19028597; 19489725; 19549727; 19690564; 19800834; 19874575; 20098290; 20360068; 20587519; 20628057; 20711500; 21378310; 21516116; 21576364; 21693106; 22493164; 23750007; 24259665; 24519943; 25036637; 25355637; 25416956; 25519010; 26088461; 26464195; 26496610; 26514267; 26612725; 26846102; 26963794; 27323192; 27648936; 28481871; 28871945; 29180353; 29410345; 30367623; |
| Motif | |
| Gene Encoded By | |
| Mass | 35,905 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |