Detail Information for IndEnz0002001745
IED ID IndEnz0002001745
Enzyme Type ID protease001745
Protein Name Extracellular elastase
EC 3.4.24.-
SEPP1
Gene Name sepA SE_2219
Organism Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus Staphylococcus epidermidis Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200)
Enzyme Sequence MKNFSKFALTSIAALTVASPLVNTEVDAKDKVSATQNIDAKVTQESQATNALKELPKSENIKKHYKDYKVTDTEKDNKGFTHYTLQPKVGNTYAPDKEVKVHTNKEGKVVLVNGDTDAKKVQPTNKVAISKESATDKAFEAIKIDRQKAKNLKSDVIKTNKVEIDGEKNKYVYNIEIITTSPKISHWNVKIDAETGQVVDKLNMIKEAATTGTGKGVLGDTKQININSVSGGYALQDLTQQGTLSAYNYDANTGQAYLMQDKDKNFVDDEQRAGVDANYYAKETYDYYKNTFGRESYDNQGSPIISIAHVNNFQGQDNRNNAAWIGDKMIYGDGDGRTFTALSGANDVVAHEITHGVTQQTANLVYRSQSGALNESFSDVFGYFIDDEDFLMGEDVYTPGVGGDALRSMSNPERFGQPSHMNDFVYTNSDNGGVHTNSGIPNKAAYNTIRSIGKQRSEQIYYRALTVYLTSNSDFQDAKASLQQAAFDLYGDGIAQQVGQAWDSVGV
Enzyme Length 507
Uniprot Accession Number P0C0Q4
Absorption
Active Site ACT_SITE 352; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; ACT_SITE 435; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.24.-
Enzyme Function FUNCTION: Protease that has a low substrate specificity. Glucagon is preferentially cleaved between aromatic (Phe) and hydrophobic (Val) amino acids. Hydrolyzes casein and elastin (By similarity). {ECO:0000250}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Metal binding (16); Propeptide (1); Signal peptide (1)
Keywords Calcium;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc;Zymogen
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..28; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 55,787
Kinetics
Metal Binding METAL 347; /note=Calcium 1; /evidence=ECO:0000250; METAL 351; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 355; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 375; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 386; /note=Calcium 1; /evidence=ECO:0000250; METAL 386; /note=Calcium 2; /evidence=ECO:0000250; METAL 388; /note=Calcium 2; /evidence=ECO:0000250; METAL 389; /note=Calcium 1; /evidence=ECO:0000250; METAL 389; /note=Calcium 2; /evidence=ECO:0000250; METAL 391; /note=Calcium 1; via carbonyl oxygen; /evidence=ECO:0000250; METAL 394; /note=Calcium 1; /evidence=ECO:0000250; METAL 394; /note=Calcium 2; /evidence=ECO:0000250; METAL 397; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 398; /note=Calcium 3; /evidence=ECO:0000250; METAL 401; /note=Calcium 3; via carbonyl oxygen; /evidence=ECO:0000250; METAL 404; /note=Calcium 3; /evidence=ECO:0000250
Rhea ID
Cross Reference Brenda