| IED ID | IndEnz0002001751 |
| Enzyme Type ID | protease001751 |
| Protein Name |
Seminal metalloprotease 1 EC 3.4.24.- |
| Gene Name | Semp1 CG11864 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MFPQIWGVIFLFTPTVFSELFKDPELLAGFYQGDIKAHPIRTRNGIVNQIYHWPNRTVPYMIEDDAFADSHYREILRAISIIEENSCVIFKPATEMDFPMALVITSKGLGCNTVHLGYRNKTQVVNLEIYPLGEGCFRIGSIIHELLHVLGFEHQHVSQNRDQYVSIQWKNINPQYNINFVNNDNSTAWHDFDEGYDYESVMHYVPRAFSRNGQPTIVPLREGAENMGQRFYMSEKDIRKLNKMYRCPDHV |
| Enzyme Length | 251 |
| Uniprot Accession Number | Q9VJN9 |
| Absorption | |
| Active Site | ACT_SITE 145; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.24.- |
| Enzyme Function | FUNCTION: Seminal fluid metalloprotease which is transferred to females during mating and is required for processing of two other seminal fluid proteins Acp26Aa and Acp36DE in mated females. {ECO:0000269|PubMed:15979005, ECO:0000269|PubMed:17116868, ECO:0000269|PubMed:24514904}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Disulfide bond (2); Domain (1); Erroneous initiation (1); Glycosylation (3); Metal binding (3); Mutagenesis (3); Propeptide (1); Sequence conflict (3); Signal peptide (1) |
| Keywords | Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Reference proteome;Secreted;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15979005, ECO:0000269|PubMed:17116868}. Note=Secreted into seminal fluid. {ECO:0000269|PubMed:15979005, ECO:0000269|PubMed:17116868}. |
| Modified Residue | |
| Post Translational Modification | PTM: Undergoes cleavage in the male during mating with a cleaved product detected in the ejaculatory duct and/or bulb of males by 8-10 minutes after the start of mating (PubMed:17116868). Further cleavage occurs in the mated female (PubMed:15979005). May undergo cleavage in a two-step process where it is first cleaved by Sems, making it susceptible to activational cleavage which may be carried out by another protease or by autocleavage (PubMed:24514904). {ECO:0000269|PubMed:15979005, ECO:0000269|PubMed:17116868, ECO:0000269|PubMed:24514904}. |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10471707; 10779493; 15345744; 15944345; 17110486; 18056920; 18245332; 18666829; 20220848; 21074052; 21672851; 21940639; 22253601; 23071443; 23087839; 23092796; 23109270; 23555301; 23944235; 24973093; 25117438; 27172210; 27357258; 31722958; 32611817; |
| Motif | |
| Gene Encoded By | |
| Mass | 29,282 |
| Kinetics | |
| Metal Binding | METAL 144; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211; METAL 148; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211; METAL 154; /note=Zinc; via tele nitrogen; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU01211 |
| Rhea ID | |
| Cross Reference Brenda |