| IED ID | IndEnz0002001754 |
| Enzyme Type ID | protease001754 |
| Protein Name |
Tricorn protease homolog 1 EC 3.4.21.- |
| Gene Name | tri1 SCO2549 SCC77.16c |
| Organism | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces albidoflavus group Streptomyces coelicolor Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) |
| Enzyme Sequence | MGVTQPAAPAYLRFPHPHGELVAFTAEDDVWLAPLDGGRAWRVSADNVPVNHPRISPDGTKVAWTSTRDGAPEVHVAPVEGGPAKRLTHWGSIRTQVRGWTADGRVLALSTYGQASLRRSWARALPLDGGPATTLPYGPVGDVAQGPHTVLLSAPMGREAAWWKRYRGGTAGKLWIDREDDGEFVRLHDGLDGNIEYPFWVGDRIAFLSDHEGTGALYSSLADGSDLRRHTPVDGFYARHAATDGSRVVYASAGELWTLDDLDGAEPRRLDIRLGGARVDLQSYPVNAARWFGSASPDHTARGSAVAVRGGVHWVTHRSGPARALAATPGVRNRLPRTFRVDGEEWVVWVTDAEGDDALEFAPATGLAPGATARRLAAGQLGRVLHLAVAPDGSRVAVASHDGRVLLVERESGEVREVDRSEDGDASGLVFSPDSSWLAWSHPGPEPLRQLKLANTTDLSVSEATPLRFKDYSPAFTLDGKHLAFLSTRSFDPVYDEHVFDLAFVEGARPYLITLAATTPSPFGPQRHGRPFETPDREETPDSEGTPTTRIDIEGLADRIVPFPVEAARYSRLRAAKDGVLWLRHPLTGVLGASRANPEDPDPNTELERYDLAQQRVEHLGGDADHFEVSGDGKRVLLWTDGRLKVVPSDRRASGDEDSDTNITVDLGRVRQTVEPAAEWRQMFDETGRIMRDHYWRADMNGVDWDGVLDRYRPVLDRVATHDDLVDLLWEVHGELGTSHAYVTPRGGHGSGARQGLLGADLSRHEDGAWRIDRVLPSETSDPDARSPLAAPGVAVRAGDAIVAVAGQAVDPVTGPGPLLVGTAGKPVELTVSPSGGGEVRHAVVVPLADEEPLRYHAWVADRRAYVHEKSGGRLGYLHVPDMQAPGWAQIHRDLRVEVAREGLVVDVRENRGGHTSQLVVEKLARRIVGWDLPRGMRPTSYPQDAPRGPVVAVANEFSGSDGDIVNAAIKALGIGPVVGVRTWGGVIGIDSRYRLVDGTLITQPKYAFWLEGYGWGVENHGVDPDVEVPQRPQDHAAGRDPQLDEAIALALAALEETPAKTPPSLP |
| Enzyme Length | 1067 |
| Uniprot Accession Number | Q9RDE2 |
| Absorption | |
| Active Site | ACT_SITE 740; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 961; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P96086; ACT_SITE 1019; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P96086 |
| Activity Regulation | ACTIVITY REGULATION: Stimulated by MgCl2. |
| Binding Site | BINDING 914; /note=Substrate; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:P96086 |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Degrades oligopeptides in a sequential manner. {ECO:0000250}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 37 degrees Celsius.; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5-7.8.; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Binding site (1); Chain (1); Compositional bias (1); Region (2); Site (1) |
| Keywords | Cytoplasm;Hydrolase;Protease;Reference proteome;Serine protease |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 115,647 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |