| IED ID | IndEnz0002001782 |
| Enzyme Type ID | protease001782 |
| Protein Name |
Trypsin-3 EC 3.4.21.4 Brain trypsinogen Mesotrypsin Mesotrypsinogen Serine protease 3 Serine protease 4 Trypsin III Trypsin IV |
| Gene Name | PRSS3 PRSS4 TRY3 TRY4 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MCGPDDRCPARWPGPGRAVKCGKGLAAARPGRVERGGAQRGGAGLELHPLLGGRTWRAARDADGCEALGTVAVPFDDDDKIVGGYTCEENSLPYQVSLNSGSHFCGGSLISEQWVVSAAHCYKTRIQVRLGEHNIKVLEGNEQFINAAKIIRHPKYNRDTLDNDIMLIKLSSPAVINARVSTISLPTTPPAAGTECLISGWGNTLSFGADYPDELKCLDAPVLTQAECKASYPGKITNSMFCVGFLEGGKDSCQRDSGGPVVCNGQLQGVVSWGHGCAWKNRPGVYTKVYNYVDWIKDTIAANS |
| Enzyme Length | 304 |
| Uniprot Accession Number | P35030 |
| Absorption | |
| Active Site | ACT_SITE 120; /note="Charge relay system"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00274, ECO:0000305|PubMed:27810896"; ACT_SITE 164; /note="Charge relay system"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00274, ECO:0000305|PubMed:27810896"; ACT_SITE 257; /note="Charge relay system"; /evidence="ECO:0000255|PROSITE-ProRule:PRU00274, ECO:0000305|PubMed:27810896" |
| Activity Regulation | ACTIVITY REGULATION: Not inhibited by Kunitz-type trypsin inhibitors. {ECO:0000269|PubMed:18077447, ECO:0000269|PubMed:9099703}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; Evidence={ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:14507909, ECO:0000269|PubMed:18077447, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0000269|PubMed:6698368, ECO:0000269|PubMed:9099703}; |
| DNA Binding | |
| EC Number | 3.4.21.4 |
| Enzyme Function | FUNCTION: Digestive protease that cleaves proteins preferentially after an Arg residue and has proteolytic activity toward Kunitz-type trypsin inhibitors. {ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:14507909, ECO:0000269|PubMed:18077447, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0000269|PubMed:9099703}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:6698368}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Alternative sequence (4); Beta strand (17); Chain (1); Disulfide bond (5); Domain (1); Helix (4); Metal binding (5); Modified residue (1); Mutagenesis (1); Natural variant (4); Propeptide (1); Sequence conflict (3); Signal peptide (1); Site (1); Turn (3) |
| Keywords | 3D-structure;Alternative splicing;Calcium;Digestion;Disulfide bond;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Sulfation;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:6698368}. |
| Modified Residue | MOD_RES 211; /note=Sulfotyrosine; /evidence=ECO:0000250 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..?; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (14) |
| Cross Reference PDB | 1H4W; 2R9P; 3L33; 3L3T; 3P92; 3P95; 4DG4; 4U30; 4U32; 5C67; 5JBT; 5TP0; 6BX8; 6GFI; |
| Mapped Pubmed ID | 15174051; 15299926; 15572765; 15855826; 15987713; 16013053; 16412431; 16492676; 16759229; 17406981; 17623652; 18094465; 18363639; 18665091; 19095652; 19249338; 19254208; 19920152; 20035377; 20057044; 20124702; 20484962; 20711500; 20861008; 20947888; 21806544; 22042864; 22547309; 22610453; 22900303; 23650620; 24146905; 24189400; 24872419; 25609649; 26175157; 26318044; 26957636; 28423522; 28463992; 28844099; 29629340; 30258049; 30700553; 30755669; 30908656; 31810373; 31911942; 32014997; 33515547; |
| Motif | |
| Gene Encoded By | |
| Mass | 32,529 |
| Kinetics | |
| Metal Binding | METAL 132; /note="Calcium"; /evidence="ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0007744|PDB:1H4W, ECO:0007744|PDB:3L33, ECO:0007744|PDB:3L3T, ECO:0007744|PDB:3P92, ECO:0007744|PDB:3P95, ECO:0007744|PDB:4U30, ECO:0007744|PDB:4U32, ECO:0007744|PDB:5JBT, ECO:0007744|PDB:5TP0"; METAL 134; /note="Calcium; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0007744|PDB:1H4W, ECO:0007744|PDB:3L33, ECO:0007744|PDB:3L3T, ECO:0007744|PDB:3P92, ECO:0007744|PDB:3P95, ECO:0007744|PDB:4U30, ECO:0007744|PDB:4U32, ECO:0007744|PDB:5JBT, ECO:0007744|PDB:5TP0"; METAL 137; /note="Calcium; via carbonyl oxygen"; /evidence="ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0007744|PDB:1H4W, ECO:0007744|PDB:3L33, ECO:0007744|PDB:3L3T, ECO:0007744|PDB:3P92, ECO:0007744|PDB:3P95, ECO:0007744|PDB:4U30, ECO:0007744|PDB:4U32, ECO:0007744|PDB:5JBT, ECO:0007744|PDB:5TP0"; METAL 139; /note="Calcium"; /evidence="ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0007744|PDB:1H4W, ECO:0007744|PDB:3L33, ECO:0007744|PDB:3L3T, ECO:0007744|PDB:3P92, ECO:0007744|PDB:3P95, ECO:0007744|PDB:4U30, ECO:0007744|PDB:4U32, ECO:0007744|PDB:5JBT"; METAL 142; /note="Calcium"; /evidence="ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0007744|PDB:1H4W, ECO:0007744|PDB:3L33, ECO:0007744|PDB:3L3T, ECO:0007744|PDB:3P92, ECO:0007744|PDB:3P95, ECO:0007744|PDB:4U32, ECO:0007744|PDB:5JBT, ECO:0007744|PDB:5TP0" |
| Rhea ID | |
| Cross Reference Brenda |