| IED ID | IndEnz0002001784 |
| Enzyme Type ID | protease001784 |
| Protein Name |
Trypsin-3 EC 3.4.21.4 Trypsin III Fragment |
| Gene Name | |
| Organism | Salmo salar (Atlantic salmon) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Euteleosteomorpha Protacanthopterygii Salmoniformes (salmons and trouts) Salmonidae (salmonids) Salmoninae (trouts salmons & chars) Salmo Salmo salar (Atlantic salmon) |
| Enzyme Sequence | FAVAFAAPIDDEDDKIVGGYECRKNSASYQASLQSGYHFCGGSLISSTWVVSAAHCYKSRIQVRLGEHNIAVNEGTEQFIDSVKVIMHPSYNSRNLDNDIMLIKLSKPASLNSYVSTVALPSSCASSGTRCLVSGWGNLSGSSSNYPDTLRCLDLPILSSSSCNSAYPGQITSNMFCAGFMEGGKDSCQGDSGGPVVCNGQLQGVVSWGYGCAQRNKPGVYTKVCNYRSWISSTMSSN |
| Enzyme Length | 238 |
| Uniprot Accession Number | P35033 |
| Absorption | |
| Active Site | ACT_SITE 55; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 99; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 192; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; |
| DNA Binding | |
| EC Number | 3.4.21.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (14); Chain (1); Disulfide bond (6); Domain (1); Helix (4); Metal binding (4); Non-terminal residue (1); Propeptide (1); Signal peptide (1); Site (1); Turn (3) |
| Keywords | 3D-structure;Calcium;Digestion;Disulfide bond;Hydrolase;Metal-binding;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL <1..7; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1A0J; |
| Mapped Pubmed ID | 9757092; |
| Motif | |
| Gene Encoded By | |
| Mass | 25,389 |
| Kinetics | |
| Metal Binding | METAL 67; /note=Calcium; /evidence=ECO:0000250; METAL 69; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 72; /note=Calcium; via carbonyl oxygen; METAL 77; /note=Calcium; /evidence=ECO:0000250 |
| Rhea ID | |
| Cross Reference Brenda |