| IED ID | IndEnz0002001807 |
| Enzyme Type ID | protease001807 |
| Protein Name |
Subtilisin EC 3.4.21.62 |
| Gene Name | sub1 |
| Organism | Bacillus sp. (strain TA39) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus unclassified Bacillus (in: Bacteria) Bacillus sp. (strain TA39) |
| Enzyme Sequence | MKRSGKIFTTAMLAVTLMMPAMGVSANEGNAAAEGNEKFRVLVDSVDQKNLKNAKQQYGVHWDFAGEGFTTDMNEKQFNALKKNKNLTVEKVPELEIATATDKPEALYNAMAASQSTPWGIKAIYNNSSITQTSGGGGINIAVLDTGVNTNHPDLRNNVEQCKDFTVGTTYTNNSCTDRQGHGTHVAGSALADGGTGNGVYGVAPDADLWAYKVLGDDGSGYADDIAAAIRHAGDQATALNTKVVINMSLGSSGESSLITNAVNYSYNKGVLIIAAAGNSGPYQGSIGYPGALVNAVAVAALENKVENGTYRVADFSSRGYSWTDGDYAIQKGDVEISAPGAAIYSTWFDGGYATISGTSMASPHAAGLAAKIWAQYPSASNVDVRGELQYRAYENDILSGYYAGYGDDFASGFGFATVQ |
| Enzyme Length | 420 |
| Uniprot Accession Number | P28842 |
| Absorption | |
| Active Site | ACT_SITE 145; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 182; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 360; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.; EC=3.4.21.62; |
| DNA Binding | |
| EC Number | 3.4.21.62 |
| Enzyme Function | FUNCTION: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Still active at temperatures close to 0 degrees Celsius. Thermolabile.; |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Metal binding (2); Propeptide (1); Signal peptide (1) |
| Keywords | Calcium;Hydrolase;Metal-binding;Protease;Secreted;Serine protease;Signal;Sporulation;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..31; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 44,087 |
| Kinetics | |
| Metal Binding | METAL 115; /note=Calcium; /evidence=ECO:0000255; METAL 154; /note=Calcium; /evidence=ECO:0000255 |
| Rhea ID | |
| Cross Reference Brenda |