IED ID | IndEnz0002001841 |
Enzyme Type ID | protease001841 |
Protein Name |
Peptidyl-Lys metalloendopeptidase MEP EC 3.4.24.20 AmMEP |
Gene Name | MEP |
Organism | Armillaria mellea (Shoestring root rot fungus) (Honey mushroom) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Basidiomycota Agaricomycotina Agaricomycetes Agaricomycetidae Agaricales Physalacriaceae Armillaria Armillaria mellea (Shoestring root rot fungus) (Honey mushroom) |
Enzyme Sequence | MFSLSSRFFLYSLCLSAVAVSAAPGLSLSLSGADSVVDVENLNVAATLTNTGDTTLKILNDPSSILSSKFATHTFDISSDNGSPAFTGVKVKYDPNYVVKKNADSSFTVLAPGESVTVNHALGAAYNFTGSGAASYSIEPSSLFYYVDPDTNELASINADTQQHTTKISGTLAVARRSNLGKRISYNGCTSSRQTTLVSAAAAAQTYAQSSYNYLSSHTASTTRYVTWFGPYTSARHSTVLSCFSNMLAYPYANYEYDCTCTESDVYAYVYPSQFGTIYLCGAFWQTTTTGTDSRGGTLIHESSHFTIICGTQDYAYGQSAAKSLASSNPSEAIKNADNYEYFAENNPAQS |
Enzyme Length | 351 |
Uniprot Accession Number | Q9Y7F7 |
Absorption | |
Active Site | ACT_SITE 302; /evidence=ECO:0000250|UniProtKB:P81054 |
Activity Regulation | ACTIVITY REGULATION: Inhibited by chelating agents such as imidazole, alpha,alpha'-bipyridine, and 1,10-phenanthroline. {ECO:0000269|PubMed:23849}. |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Preferential cleavage in proteins: -Xaa-|-Lys- (in which Xaa may be Pro).; EC=3.4.24.20; |
DNA Binding | |
EC Number | 3.4.24.20 |
Enzyme Function | |
Temperature Dependency | |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7-7.5. Active from pH 5.25 to pH 9. {ECO:0000269|PubMed:23849}; |
Pathway | |
nucleotide Binding | |
Features | Active site (1); Chain (1); Disulfide bond (2); Metal binding (3); Propeptide (1); Sequence conflict (2); Signal peptide (1); Site (1) |
Keywords | Cleavage on pair of basic residues;Direct protein sequencing;Disulfide bond;Hydrolase;Metal-binding;Metalloprotease;Protease;Secreted;Signal;Zinc;Zymogen |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Note=Binds strongly to beta-1,3-glucan and chitin, major polysaccharides constituting the fungal cell wall. {ECO:0000250}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 37,551 |
Kinetics | |
Metal Binding | METAL 301; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P81054; METAL 305; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P81054; METAL 314; /note=Zinc; catalytic; /evidence=ECO:0000250|UniProtKB:P81054 |
Rhea ID | |
Cross Reference Brenda | 3.4.24.20; |