| IED ID | IndEnz0002001873 |
| Enzyme Type ID | protease001873 |
| Protein Name |
Squamous cell carcinoma antigen recognized by T-cells 3 SART-3 Tat-interacting protein of 110 kDa Tip110 p110 nuclear RNA-binding protein |
| Gene Name | SART3 KIAA0156 TIP110 |
| Organism | Homo sapiens (Human) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human) |
| Enzyme Sequence | MATAAETSASEPEAESKAGPKADGEEDEVKAARTRRKVLSRAVAAATYKTMGPAWDQQEEGVSESDGDEYAMASSAESSPGEYEWEYDEEEEKNQLEIERLEEQLSINVYDYNCHVDLIRLLRLEGELTKVRMARQKMSEIFPLTEELWLEWLHDEISMAQDGLDREHVYDLFEKAVKDYICPNIWLEYGQYSVGGIGQKGGLEKVRSVFERALSSVGLHMTKGLALWEAYREFESAIVEAARLEKVHSLFRRQLAIPLYDMEATFAEYEEWSEDPIPESVIQNYNKALQQLEKYKPYEEALLQAEAPRLAEYQAYIDFEMKIGDPARIQLIFERALVENCLVPDLWIRYSQYLDRQLKVKDLVLSVHNRAIRNCPWTVALWSRYLLAMERHGVDHQVISVTFEKALNAGFIQATDYVEIWQAYLDYLRRRVDFKQDSSKELEELRAAFTRALEYLKQEVEERFNESGDPSCVIMQNWARIEARLCNNMQKARELWDSIMTRGNAKYANMWLEYYNLERAHGDTQHCRKALHRAVQCTSDYPEHVCEVLLTMERTEGSLEDWDIAVQKTETRLARVNEQRMKAAEKEAALVQQEEEKAEQRKRARAEKKALKKKKKIRGPEKRGADEDDEKEWGDDEEEQPSKRRRVENSIPAAGETQNVEVAAGPAGKCAAVDVEPPSKQKEKAASLKRDMPKVLHDSSKDSITVFVSNLPYSMQEPDTKLRPLFEACGEVVQIRPIFSNRGDFRGYCYVEFKEEKSALQALEMDRKSVEGRPMFVSPCVDKSKNPDFKVFRYSTSLEKHKLFISGLPFSCTKEELEEICKAHGTVKDLRLVTNRAGKPKGLAYVEYENESQASQAVMKMDGMTIKENIIKVAISNPPQRKVPEKPETRKAPGGPMLLPQTYGARGKGRTQLSLLPRALQRPSAAAPQAENGPAAAPAVAAPAATEAPKMSNADFAKLFLRK |
| Enzyme Length | 963 |
| Uniprot Accession Number | Q15020 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | |
| Enzyme Function | FUNCTION: U6 snRNP-binding protein that functions as a recycling factor of the splicing machinery. Promotes the initial reassembly of U4 and U6 snRNPs following their ejection from the spliceosome during its maturation (PubMed:12032085). Also binds U6atac snRNPs and may function as a recycling factor for U4atac/U6atac spliceosomal snRNP, an initial step in the assembly of U12-type spliceosomal complex. The U12-type spliceosomal complex plays a role in the splicing of introns with non-canonical splice sites (PubMed:14749385). May also function as a substrate-targeting factor for deubiquitinases like USP4 and USP15. Recruits USP4 to ubiquitinated PRPF3 within the U4/U5/U6 tri-snRNP complex, promoting PRPF3 deubiquitination and thereby regulating the spliceosome U4/U5/U6 tri-snRNP spliceosomal complex disassembly (PubMed:20595234). May also recruit the deubiquitinase USP15 to histone H2B and mediate histone deubiquitination, thereby regulating gene expression and/or DNA repair (PubMed:24526689). May play a role in hematopoiesis probably through transcription regulation of specific genes including MYC (By similarity). {ECO:0000250|UniProtKB:Q9JLI8, ECO:0000269|PubMed:12032085, ECO:0000269|PubMed:14749385, ECO:0000269|PubMed:20595234, ECO:0000269|PubMed:24526689}.; FUNCTION: Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication. {ECO:0000269|PubMed:11959860}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (5); Beta strand (7); Chain (1); Coiled coil (3); Compositional bias (3); Domain (2); Helix (28); Initiator methionine (1); Modified residue (10); Motif (1); Natural variant (3); Region (9); Repeat (8); Turn (4) |
| Keywords | 3D-structure;Acetylation;Alternative splicing;Coiled coil;Cytoplasm;Direct protein sequencing;Methylation;Nucleus;Phosphoprotein;RNA-binding;Reference proteome;Repeat;mRNA processing;mRNA splicing |
| Interact With | Q15287; Q6ZNG0; Q9NQZ8; F5HFL9 |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Nucleus, nucleoplasm {ECO:0000269|PubMed:10463607, ECO:0000269|PubMed:11959860, ECO:0000269|PubMed:12578909}. Nucleus, Cajal body {ECO:0000269|PubMed:12578909}. Nucleus speckle {ECO:0000269|PubMed:11959860}. Cytoplasm {ECO:0000269|PubMed:10463607, ECO:0000269|PubMed:11959860}. |
| Modified Residue | MOD_RES 2; /note="N-acetylalanine"; /evidence="ECO:0000269|Ref.13, ECO:0007744|PubMed:19369195, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378"; MOD_RES 10; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163"; MOD_RES 16; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17081983"; MOD_RES 215; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:23186163"; MOD_RES 650; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:23186163"; MOD_RES 657; /note="Phosphothreonine"; /evidence="ECO:0007744|PubMed:23186163"; MOD_RES 769; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:23186163"; MOD_RES 795; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:23186163"; MOD_RES 852; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17525332"; MOD_RES 906; /note="Omega-N-methylarginine"; /evidence="ECO:0007744|PubMed:24129315" |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | NMR spectroscopy (1); X-ray crystallography (6) |
| Cross Reference PDB | 2DO4; 5CTQ; 5CTR; 5CTT; 5JJW; 5JJX; 5JPZ; |
| Mapped Pubmed ID | 11477570; 11920522; 15031286; 16713569; 17353931; 18567812; 19148489; 19367725; 19615732; 20360068; 20711500; 21988832; 22132941; 22623428; 23088399; 23455153; 23487458; 23606334; 23822148; 23834120; 24217245; 25604459; 25609649; 25928721; 25939381; 26496610; 27060135; 27255711; 28088760; 28878014; 29583087; 31619517; 31956895; 32495170; 32941384; |
| Motif | MOTIF 601..617; /note=Nuclear localization signal; /evidence=ECO:0000255 |
| Gene Encoded By | |
| Mass | 109,935 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |