| IED ID | IndEnz0002001886 |
| Enzyme Type ID | protease001886 |
| Protein Name |
Subtilisin-like protease Subtilase EC 3.4.21.- |
| Gene Name | SBT MTR_5g011320 |
| Organism | Medicago truncatula (Barrel medic) (Medicago tribuloides) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade Hologalegina IRL clade Trifolieae Medicago (medics) Medicago truncatula (Barrel medic) (Medicago tribuloides) |
| Enzyme Sequence | MMKMELRLLVSLIFILCSISMLAAEENLEHDQINLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQTFPHKERMVFSYRKVASGFAVKLTPEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTGIFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGARNLVKSAIQEPPFENFFHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEAPWILTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQLLPLVYPGSFGYGNQTQNQSLCLPGSLKNIDLSGKVVLCDVGNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAHVLPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIGDSLAPSVVYFSSRGPSQESPGILKPDIIGPGVNILAAWAVSVDNKIPAFDIVSGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYLCGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANSTYKVELEVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSDRHAVRIPISVIFK |
| Enzyme Length | 748 |
| Uniprot Accession Number | G7KEU7 |
| Absorption | |
| Active Site | ACT_SITE 147; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 206; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240; ACT_SITE 533; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU01240 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Required for arbuscular mycorrhiza (AM) development during AM symbiosis with AM fungi (e.g. Glomeromycota intraradices). {ECO:0000250|UniProtKB:A9QY40}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (3); Glycosylation (5); Sequence conflict (1); Signal peptide (1) |
| Keywords | Apoplast;Glycoprotein;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal |
| Interact With | |
| Induction | INDUCTION: Accumulates in mycorrhizal roots upon colonization by the arbuscular mycorrhiza (AM) fungus Glomus versiforme. {ECO:0000269|PubMed:12953114}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast {ECO:0000250|UniProtKB:A9QY40}. Note=Accumulates in the intercellular spaces and the periarbuscular space (PAS) during arbuscular mycorrhizal (AM) symbiosis. {ECO:0000250|UniProtKB:A9QY40}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 80,476 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |