| IED ID |
IndEnz0002001901 |
| Enzyme Type ID |
protease001901 |
| Protein Name |
Kinetochore protein SPC25
|
| Gene Name |
SPC25 YER018C |
| Organism |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage |
cellular organisms
Eukaryota
Opisthokonta
Fungi
Dikarya
Ascomycota
saccharomyceta
Saccharomycotina (true yeasts)
Saccharomycetes
Saccharomycetales
Saccharomycetaceae
Saccharomyces
Saccharomyces cerevisiae (Baker's yeast)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
|
| Enzyme Sequence |
MASIDAFSDLERRMDGFQKDVAQVLARQQNHARQQLQQFQAEMRQLHNQHQHLIDELQRLATQRTALQQQIHAAQQATNTTREQWRSYHERESELSRRQSTLAAQSRELDSLLQQRGKECVQLRARWAAQSGNDAAEVALYERLLQLRVLPGASDVHDVRFVFGDDSRCWIEVAMHGDHVIGNSHPALDPKSRATLEHVLTVQGDLAAFLVVARDMLLASL |
| Enzyme Length |
221 |
| Uniprot Accession Number |
P40014 |
| Absorption |
|
| Active Site |
|
| Activity Regulation |
|
| Binding Site |
|
| Calcium Binding |
|
| catalytic Activity |
|
| DNA Binding |
|
| EC Number |
|
| Enzyme Function |
FUNCTION: Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity. {ECO:0000269|PubMed:11266451, ECO:0000269|PubMed:12514103}. |
| Temperature Dependency |
|
| PH Dependency |
|
| Pathway |
|
| nucleotide Binding |
|
| Features |
Beta strand (6); Chain (1); Coiled coil (1); Helix (3); Initiator methionine (1); Modified residue (1); Region (1) |
| Keywords |
3D-structure;Acetylation;Cell cycle;Cell division;Centromere;Chromosome;Coiled coil;Kinetochore;Mitosis;Nucleus;Reference proteome |
| Interact With |
P40460; P33895; Q04477 |
| Induction |
|
| Subcellular Location |
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11179222}. Chromosome, centromere, kinetochore {ECO:0000269|PubMed:11179222, ECO:0000269|PubMed:11266451}. Note=Associated with kinetochores. {ECO:0000269|PubMed:11179222}. |
| Modified Residue |
MOD_RES 2; /note=N-acetylalanine; /evidence=ECO:0007744|PubMed:22814378 |
| Post Translational Modification |
|
| Signal Peptide |
|
| Structure 3D |
NMR spectroscopy (1); X-ray crystallography (6) |
| Cross Reference PDB |
2FTX;
2FV4;
4GEQ;
5T6J;
5TCS;
5TD8;
7KDF;
|
| Mapped Pubmed ID |
11087867;
11511347;
11533725;
11696340;
11805837;
12408861;
12600307;
12781157;
14565975;
14570580;
14616062;
14690591;
15521820;
15661517;
15887295;
16205707;
16429126;
16554755;
16765893;
17362199;
17521635;
17766118;
17981694;
18286174;
18491064;
18719252;
19269365;
19300438;
19467206;
19536198;
19893618;
20479465;
20479468;
20489023;
20819936;
20930138;
21075115;
21179020;
21271286;
21467141;
21862320;
22445183;
22505256;
22561345;
22561346;
22561349;
22870406;
23039831;
23058804;
23154893;
23267104;
23334295;
23512483;
23908374;
24666101;
24763395;
24930965;
25400627;
25874377;
26157162;
26877204;
26908646;
26974660;
27851957;
27881300;
33591274;
9153752;
9585415;
|
| Motif |
|
| Gene Encoded By |
|
| Mass |
25,244 |
| Kinetics |
|
| Metal Binding |
|
| Rhea ID |
|
| Cross Reference Brenda |
|