| IED ID | IndEnz0002001905 |
| Enzyme Type ID | protease001905 |
| Protein Name |
Serine proteinase stubble EC 3.4.21.- Protein stubble-stubbloid Cleaved into: Serine proteinase stubble non-catalytic chain; Serine proteinase stubble catalytic chain |
| Gene Name | Sb Sb-sbd CG4316 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MKQPTLIRPRLRHRRSTPAAATKMCPKRHWLVNNRAAGSRGSGGAAARSRRSLDQIVEVLVALIVVNCLATAAAALITPPDSLESLGSLGIPSSSASSSEDDDDMSSGFYRIPHRLEGYPQLQQLQRGQNFKISPKPCSFGRVEGTCMFVWECIKSEGKHVGMCVDSFMFGSCCTHNYTDNIVLPQTAFSYTRPTKPLTLRPRPPAAPYKPMISGMTTIERPHGAGTLVIRPSGPHHQGTLARPHPPPYQSKPTTASDLHGSASHPSSSSSSSSSSNPNSIWHTSTQQQQQQQHQQNQQNHWQMTTEPSFITKPRPTGWTKPGIVNLPMPARPSKPSKPTKKPIVYDRTPPPPPSVPPSTSTSTTSTSLIWPAQTHPPQPHRPTRPQLSPGTSLAASSSSHWPSSTTSTTSSTTSTTTTTTTTRRTTTPTTTTRRTTTNKPTRPYQRPTTATSSSSTSTTSSKTPTTTRPISSSSSSSSGIVTSSQRPTQPTHRTPVLATSGIETNEISDSSIPDAGALGHVKTISAARSECGVPTLARPETRIVGGKSAAFGRWPWQVSVRRTSFFGFSSTHRCGGALINENWIATAGHCVDDLLISQIRIRVGEYDFSHVQEQLPYIERGVAKKVVHPKYSFLTYEYDLALVKLEQPLEFAPHVSPICLPETDSLLIGMNATVTGWGRLSEGGTLPSVLQEVSVPIVSNDNCKSMFMRAGRQEFIPDIFLCAGYETGGQDSCQGDSGGPLQAKSQDGRFFLAGIISWGIGCAEANLPGVCTRISKFTPWILEHVR |
| Enzyme Length | 787 |
| Uniprot Accession Number | Q05319 |
| Absorption | |
| Active Site | ACT_SITE 590; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 640; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 738; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.21.- |
| Enzyme Function | FUNCTION: Hormone dependent protease required for epithelial morphogenesis, including the formation of bristles, legs, and wings. Has a dual function, detaches imaginal disk cells from extracellular matrices through its extracellular proteolytic domain and transmits an outside-to-inside signal to its intracellular domain to modify the cytoskeleton during morphogenesis. {ECO:0000269|PubMed:7685111}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (2); Compositional bias (5); Disulfide bond (4); Domain (1); Glycosylation (2); Region (2); Sequence conflict (4); Topological domain (2); Transmembrane (1) |
| Keywords | Disulfide bond;Glycoprotein;Hydrolase;Membrane;Protease;Reference proteome;Serine protease;Signal-anchor;Transmembrane;Transmembrane helix;Zymogen |
| Interact With | |
| Induction | INDUCTION: By 20-hydroxyecdysone (20HE). {ECO:0000269|PubMed:7685111}. |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein. |
| Modified Residue | |
| Post Translational Modification | PTM: May activate itself by proteolytic cleavage. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10569241; 10655219; 10961941; 11060317; 11106377; 11156609; 11274417; 119666; 12070612; 12136022; 12235290; 12364793; 12548287; 12568721; 12710964; 1331746; 14517310; 1459427; 14668390; 14668391; 14942737; 14972681; 15204079; 15371540; 1541393; 15901829; 15998724; 16322506; 16357198; 16933974; 17246118; 17246347; 17247021; 17248400; 17462898; 17625558; 17970746; 17996400; 18221380; 1903118; 19196654; 19692395; 19852616; 19862331; 19948966; 2004417; 2015798; 20220848; 20371351; 20505245; 20573980; 20662923; 21074052; 2127579; 2155155; 22227521; 22276549; 22662148; 2275822; 23071443; 23166712; 23720823; 23749451; 23850418; 24550732; 2501647; 25312911; 25644700; 26453810; 26551273; 26656493; 27172211; 28067623; 29974861; 30367934; 3143619; 31683705; 32122911; 32898719; 32993647; 33115752; 33193649; 33827210; 402295; 5003954; 6050992; 6434989; 7607426; 7661850; 7707961; 7720077; 7876156; 7982560; 8001783; 8005411; 8020105; 811500; 8417992; 8643671; 8807299; 9093849; 9094207; 9258681; 9295137; 9326622; 9475733; 9521911; 9601646; 9654025; 9728921; 9891050; |
| Motif | |
| Gene Encoded By | |
| Mass | 85,144 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |