| IED ID | IndEnz0002001928 |
| Enzyme Type ID | protease001928 |
| Protein Name |
Replicase polyprotein 1ab pp1ab ORF1ab polyprotein Cleaved into: Non-structural protein 1 nsp1 EC 3.4.22.- Papain-like proteinase PL-PRO ; Non-structural protein 2 nsp2 ; 3C-like serine proteinase 3CLSP EC 3.4.21.- M-PRO nsp3 p27 ; Non-structural protein 4 nsp4 ; Non-structural protein 5 nsp5 ; Non-structural protein 6 nsp6 ; Non-structural protein 7 nsp7 ; Non-structural protein 8 nsp8 ; RNA-directed RNA polymerase Pol RdRp EC 2.7.7.48 nsp10 p100 ; Helicase Hel EC 3.6.4.12 EC 3.6.4.13 nsp11 p67 ; Exoribonuclease ExoN EC 3.1.13.- nsp12 ; Non-structural protein 13 nsp13 ; Uridylate-specific endoribonuclease EC 3.1.-.- NendoU nsp14 ; Putative 2'-O-methyl transferase EC 2.1.1.- nsp15 |
| Gene Name | rep 1a-1b |
| Organism | Breda virus 1 (BRV-1) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Pisuviricota Pisoniviricetes Nidovirales Tornidovirineae Tobaniviridae Torovirinae Torovirus Renitovirus Bovine torovirus Breda virus Breda virus 1 (BRV-1) |
| Enzyme Sequence | MSKTSRELTNETELHLCSSTLDLISKSQLLAQCLGTPQNLVSLSKMVPSILESPTLEPRYTSTHSSSLQSLQLLALNTSSTLYKWTTGSISKLRGHLERELCRGLVPLNDFIPKGNYVELSLMIPSVLTGQGTSTTTTLQEMCSDMVQSCIKSMETDLLKGVLALKDQTSCQEYFLSANYQSLIPPQPLVNAMRMSSVVDLSPLILENTRLLLKLSPFHGGTSVSYTSMIREFVDCSRRDEKCLKRRLTKKQKRQEEGSFDANKVITLGGKMYRYRVVILKCSDEVDDLIGFDGKVGEFDYNFENVPHCWRDLVKRRCLIRAKATWNLAGGVDENLDHVYIDESQXDFRCADGSSDSPSACVEDPHLEERIFSRVWLKQTSRFFGTKIQQVSELFKSIGLPELETTYCGVNPVKVGNKWLSFRDQGRSRVFFVYTDSNVYLATTRQKVCCDYILTKFKSVKWIGNKPDQCRVVKVLAWLISVNKVKNCTRVITPMLTVQGKISHRRVDYLDISVLDSYVSDTAGLNCVQKVKKFLSMYYNCGADLGLLDNFLTPIECGTKQLVFERCNCPNHQFYVAQFDNHVVLGLGRPTGVVYPEEIPSCANIYAVGFATQKRVVEVHYYSEMDRHQLPQDYYYFAYDQEFQHVGGDDYVNHHLDDVEDQPFPPVLFDDVYDSGDSLDDGGSDLDCFDVGYDFFWPEAPIPVPSPYGYYQGQRLRDLCVAGGDFGCDCPRCDGTFIYHPFRPRHYHSFDEVGPFIQMCEFTLTYSGQNYNLFYGLEPKVCLQDLVEASDKLLQLLVRGQLENISLPNDILACLSSLKLGANIHPFLWPAPFFNANGEWVDIFGGGDFTVFGEDFCLKAKSMVESVYFLVENFFSVDCPIGNLYCNLHLDGDVKKMLWSTIHMKYIYLALIHSEKVFNIILNSRQLSHQELVKLVIIGTFDVSIVAPCACSGDCNHGKVYNWTNLLSSVYRFVTLDQLVGLSYCEKRSLVLRKVQQYLEVEEGYQRPVQLLMAPFYGFNDNAEPDEQPLTGVFHQQVMQMFDTCVMLDVICGLKRPRASVYNLFGVLADYFRRPFTFRYYQVAEFSGSESTQVFTDVTSALTSKDPCSNRPYIYHDYAVCRVVEPRTAAVTTRGAIYPPEVIEMIRSYLPIEFDVGVMNYVDGNCDFKYCNLEFCLSGRGLVKLDTGELLDYKTNLFVVRYKTLPLLYVTSNPIYLSDFSLDNAVCLTGDFKLSFDVEPGSTLFGLYFTNGRCYRDVWETLPRFGLGTLSPPKCHSKCEPFENLAEVFFFKRRVQLVPLVNNYTPVFRHRPDIPKVLTVELMPYYSSIGYQGFVAPKCVLPGCVATQYCKLRHQLDRCVQVTKLAVAYAFYFKPLNIGSLYHLDPMRGTSYGKPAVVQFEPVGLIKEVNILVYQFGKHVAIHYFPECPTYVAYGHYPSHSVGVWLGYLPSVEECVIAQRNYRVYVPTCFRLSRTGCYHIQQDEDFERTHITVSYHYARDFDTKSLTPMFQMFSKIFGKSKQDLICALNSLSEESQSVLTLFCEEFDSAYTLQTISDEVSFETSTSPELVACVLAYAIGYELCLTVKTDGECESLDVGSSLEQVYVDYDVSKNVWDLSTHLQDDSSDDLELPFNQYYEFKVGRASVVLVQDDFKSVYDFLKSEQGVDYVVNPANNQLKHGGGIAKVISCMCGPKLTSWSNNYIKQYKKLGVTCAIRSPGFQLGKGVQIIHVVGPKSADSDVVNKLEASWRSVFQNVKPDTTVLTSMLSTGIFGCSVTDSATTLLSNLVDLDKDVVVFVVTNVSDQYIEALGVVESFQSAHGLPNFGNTCWFNALYQLLKSFAVKEQIVQDLVNCFDDFYECPTRQCVEWVCDQLGVVFGEQYDAVEMLVKIFDVFKCNVRVGYDCLARLQQVALGSCREVPADAVLMFFGQDKSGHWVAARKVCGVWYTFDDKVVVKKDPDWSKVVLVLRERGLFKATDFETPRPRRRRVAYRVPRDTISQDAIMFLEERQFSSGTMLAHSCVESVESFHVEGVQPSPLQSVDGLDDVADLSCDNHVCDNSDLQEPQVVVSQPSEVLTTSMSIECPVLENSECSVETDLNPVCEENEQVGESGIKEQDGVTTSDSQQVFSKSLDPIIKQHEVESVEPQDLPVFSQQPQVMLSMTWRDVLFQQYLGFKSDLLSLTHVNKFKIVVYLMVLWFVLLYCFSDFSLLSRFCLYVFLLWLSHVVLVVKKLDLGLVNSGGESYVLRILSSVKVPNCIAFNCDGVHWLILKLLFYSFHFYDFFVKTLVVVFQMPQLRCFTWPLLKLGFADTFLSHHILAFPTKQVSQSCLPVFGDERKYIYVPYWCKESFRTLVARAKQLTATGRTKTLDNWHYQCCSKTVKPSSCFNVRDFVFDDACNNHKHYGFFSALWFYVVFYSGFVSFWLPLMFCYCALFMCTFKNLPVNITRPIRWTVLQQVVDDLLSIITKPLFGRPACPPLSAYLTATTADEAVRASRSLLGRFCTPVGFQQPIMNVENGVAVSSLGFINPLMWPLFIVVLLDNRFVWFFNVLSYIMLPVFVIILFYFYLRKICGCVNVKGVVKNCTRHFQNFSKPLVAAGVHGNRTNFTYQPMQENWCDRHSWYCPKEEHYMTPEMAMFIKNYYNLATSPMADTIWCDYVKSVPNMTWANFKFSLFKSNETVMCGPSSHADSMLLSWYAFLHGIRFAVNPSVIDIPSQTQPIYVSSDSDDSLDKGCDVSLRPTKNKGKFKKQSVAYFSAGPVDLWYYVMLIIALGAIFVFMYSCFMVGQYVVMPRDKFFGVNPTGYSYVNAQPYLHASPPVLRNSDGMVLATPLKVPSISYSVYRLLSGHLYFTKLIVAENECTPPFGAXRLSHEFTCNDFTYILPAHLRIFGRYIMLIHPDQLHMLPFEVEHSTHTRLCYVTGTNIVECLPTFEIISPYVFVVLVAIFTIVFLFLLRMYIVMYSYFKVFTYVVFKLLFVNTVMVLFVVCLPPLVPGVVFVLALWLCDSVVFLLYLAVLSLFILPWFYVMLFVLIVGGFVFWWMMKSSDVVHLTPDGLTFNGTFEQVSKCVFPLNPLIVNRLLLDCRMSHSDLVEKSKLKTTEGKLATEMMKVFMTGETAYYQPSNFSFQSVFSKVVSPFTLHARPPMPMFRLYVYFNGQCVGTTCTGTGFAIDDSTIVTAKHLFECDDLKPTHLSVELSCRSYWCTWKEPNVLSWKFEGENAYISVENLRDFYGIDFKYLPFQQIECEFYKRMEAVTIYSIKYGSEFATQAWQTVNGHFVCCNTEGGDSGAPLVWRDSVIGVHQGLCDSFKTTLASDSKGVMMTEVKGYHVDPPVYYKPIIMSAAYNKFVADSDVSVGECTNYHNFVNEDFFSMHDELEKVSFGDKMFRYCQSLPRYLEPLHYFHVPSFWQPFKKQSVSSNVSWVVENLHFIFSVYFLVCDFVAYWWLDDPFSVVLPLFFVVQLLSTVVLKNVLFWNTSYLVTLAVTFYVHSEVAESMYLLGLFSDQIVNRVGLILVVSVMCLFVVVRVVVNVKRAIFVVVVSVLLIVVNVVLGVVQFNSLVAVCMFDIYAVFAALLTPQPVVAIMMLILFDTKCLMSFAFVVIVLSFRVFKNYKFVRVLHNLCNFDFVLTQLSLFRYRHHNQGNNPSHYEALWLFLKELYYGVQDVKYEVFSPQAGTYNVRFLTDMTEQDQLEAVEQVQRRLQRFSIVQDKNSQRLVLYSKNVDFLRSQIQHQRVLGANPFIITTLTPKDIAIDNVEVHNPSQFKPEDLQAHMWFYSKSPIFVGQVPIPTNVQTAAVLDTTYNCQDLTADEKNNVAANLQIQNAALTLSLFEECNRFLESELGDVPTLMWQSEDVVDVKQLEVQIEKLRVVLDGMQLGTSEYKATRKQINILQSQLDKALAFERKLAKFLEKVDQQQAITNETAKQLSAFKNLVKQVYESYMSSLKVRVVESNDASCLLTSTDLPRKLVLMRPITGLDNIKIVEKANGCEITAFGDTFTTGLGSNLAGLAYSSTQPLSAYPFIFNLEGIFKQQANIGYKTVECNMSSDNGSVLYKGKIVAVPSEDNPDFVVCGKGYKLDCGINVLMIPSIVRYITLNLTDHLQRQSLKPRRRLQYKQQGVRLGGVNLGEHQAFSNELISSVGYTTWVSSTVCTDKSHKHPWFVQIPSSEKDPEWFMHNTQVKNNQWVVDAKPTHWLVDADTNEQLFALALTDEEYLKAESILAKWSPITQDVECWFKDLRGYYTVSGLQPLWPVCPKKICSLKIVPIFQSQSVAYADEPTHFLSLPVVNKNFLEAFYELQEGFPGEKQVAPHISLTMLKLTEEDVAKVEDILDEMVLPNTYATITNPHMMGQYYVFEVEGLQALHDEVVSVLRQHGIACDQTRMWKPHLTIGEIKDGSVFNKFKDFGITCKLEDCDFVKLGAPKANARYEFIATLPVGDFKLLRDVWCACRHLCFQNGAYQSSRSKHYIDLATEYNAGIVKVNKSNTHSVEYKGQRFMIKRVKDQHEFALARTAFLPSIIPHHMVHQNGEWFLVRGPTTQWSLGDLVYAIWLGDQAYLDECGFVFNPSRDEFLDDANQRSYLANLLEPAILSFCEIFHCVKGCQVPYKITLDNLDLKGQLYDFGDYPCPNKVDNQSALFVLAEVWSMTRKPFPTKFAQVLAKEMNVPADFQMYFQHTLLSGKYFDKAMCLNNVRPLLRDPANLTTTPFFSQHSGVWTHFYNPIYGLVECDLEEFSNLPEVLQQLITVQGPITSAMTPAISIGEGVYAANVPPVAAAKQKIPLYDVGLCQELTDAGVDCGEAFKYFYYLSNPAGALADVCFYDYQGTGFYSPKLLAGVYDFMKRVTTCYKTDERFTYEQAKPRKSSMGINITGYQQDAVYRALGPENITKLFEYAQKAPLPFCTKIITKFALSAKARARTVSSCSFIASTIFRFAHKPVTSKMVEVAQNSQGFCLIGVSKYGLKFSKFLKDKYGAIEQFDVFGSDYTKCDRTFPLSFRALTAALLYELGGWEEDSWLYLNEVNSYMLDTMLCDGMLLNKPGGTSSGDATTAHSNTFYNYMVHYVVAFKTILSDLSDCNKVMRIAAHNAYTTGDYGVFNTLLEEQFQTNYFLNFLSDDSFIFSKPGALKIFTCENFSNKLQTILHTKVDLTKSWATTGHIEEFCSAHIIKTDGEYHFLPSRGRLLASLLILDKLSDVDIYYMRFVAILCESAVYSRYQPEFFNGLFQVFLDKVQQFRKDYCCDPCPTQLLDRQFYENLVFTSNTEVGLVDCYLENFKLQCEFKQQAGFDRVCFCCPNPAVSVCEECYVPLPLCAYCYYVHVVISSHCKIEDKFKCPCGQDDIRELFIVLNNSICIYQCRSCVESDRLRISLLSDVDQVVRLPGFKANSASIAKNGVAQLLTPVDNVDVSLDWNHQETVQQNVARIVYHSANMTQMSIEVVYVNFSLVRNDGSSAILDIPNFKCPDTSYCLFYKPGKTGVLKFTGKGTLTSCYDSNNLTWFKVTCPDFNQPWRLATCFVIQQHDAVYPPIKSTQYENVTFVMGPPGTGKTTFVYNTYLSKASPSNRFVYCAPTHRLVGDMDEKVDGAVVVSAYNDRTYRNPVWKKDDSYDVLLCTHNTLPFIKSAVLIVDEVSLIPPHVMIKILSMGFKKVVLLGDPFQLSPVYKNHKVHFKYDTFYLLQLATQKRYLTACYRCPPQILSAFSKPYCDVGVDLVSFNNKPGKFDIIVSKQLANMQDFSVLSVLSKEYPGYVILVNYRAAVDYAMQNGLGDVTTIDSSQGTTAANHLLVLFGASNFSKTINRVIVGCSRSTTHLVVVCCPELYKHFQPILNWPEPVYRYFGMEKQSDFNIIPEVASLVFCDIEFWHYKADPNSKTRTVYPGQIAVVTSQTLQLYLGVFDDAGYKSALRGLPKDVFVPPNWVWMRKHYPSFEQHAYNMQRLFKFIIDTTCGQPWFILYSCSNDLKSLKFYVEFGTNYFCSCGELAICLMRDGLYKCRNCYGNMSISKLVNCKYLDVQKERIKLQDAHDAICQQFHGDSHEALCDAVMTKCLYLASYDAAFKDTIHVKYKDLCLEIQYKITSPYVRYDGVNKRYLYRDHGAMHYFKTPKSPMQNVYRYEVGAHTEYSINICNSYEGCQSFGKTCTKCIHIHCIVEQFMADDRYRDFILVSVVKSDFVEQALSPAAKALMLTVTRVEGKSFYTSNGQRYDLYDYDLSKSVMRVVGASVKPLPLYSVVVGLGINCTVGCVLPNVPMKLKDELLSTDVPLSTLRLDLPTWYYVTWPTLSNRTSRWKLAGAQVYDCSVHIYVEATGEQPLYYLQLGNGESLRELPETLFSTGRLYNLEHDPSKNFNVQQLAIETIPKNHHVFAGDFTDVGTDIGGVHHVVALNGYKGSIIPNYVKPIATGLINVGRSVKRTTLVDVCANQLYEKVKQQIAGVKVSKVIFVNIDFQEVQFMVFAKGEDDIQTFYPQKEFIRSYYEWPTILPELESHYDLKNYGQDPQFMPQPVNFAKYTQICTFIQEHVKVARNSLIWHVGAAGIDGCSPGDIVLSSFFKECLVYSWDVKDYDTLLEKHNYDCNFRPNLIVSDVYNVSSNVSEVLEDCVHRLALGGTIIFKTTESSRPDIQLSKVTKYFAAVHFFTAGVNTSSSEVFVVLKYKLHSEPIGEELCSPNILRRIAAYRNKLCIVPNFKVFSTSLSYRFSSVKFVQKCFYVSVPRQFCASGLIQEVPLLCQMKH |
| Enzyme Length | 6733 |
| Uniprot Accession Number | P0C6V8 |
| Absorption | |
| Active Site | ACT_SITE 3184; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000255; ACT_SITE 3222; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000255; ACT_SITE 3291; /note=Charge relay system; for 3C-like serine proteinase activity; /evidence=ECO:0000255; ACT_SITE 5860; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 5862; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 5961; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6037; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6042; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; ACT_SITE 6363; /evidence=ECO:0000255|PROSITE-ProRule:PRU01303; ACT_SITE 6380; /evidence=ECO:0000255|PROSITE-ProRule:PRU01303; ACT_SITE 6412; /evidence=ECO:0000255|PROSITE-ProRule:PRU01303; ACT_SITE 6509; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300; ACT_SITE 6585; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300; ACT_SITE 6613; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300; ACT_SITE 6647; /evidence=ECO:0000255|PROSITE-ProRule:PRU01300 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CATALYTIC ACTIVITY: Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
| DNA Binding | |
| EC Number | 3.4.22.-; 3.4.21.-; 2.7.7.48; 3.6.4.12; 3.6.4.13; 3.1.13.-; 3.1.-.-; 2.1.1.- |
| Enzyme Function | FUNCTION: The 3C-like serine proteinase is responsible for the majority of cleavages. {ECO:0000250}.; FUNCTION: The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. {ECO:0000250}.; FUNCTION: The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction. {ECO:0000250}.; FUNCTION: NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond. {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (15); Chain (14); Domain (10); Metal binding (16); Region (3); Site (12); Transmembrane (17) |
| Keywords | ATP-binding;Endonuclease;Exonuclease;Helicase;Host membrane;Hydrolase;Membrane;Metal-binding;Methyltransferase;Nuclease;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Repeat;Ribosomal frameshifting;Thiol protease;Transferase;Transmembrane;Transmembrane helix;Viral RNA replication;Zinc;Zinc-finger |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: [Non-structural protein 1]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Non-structural protein 2]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.; SUBCELLULAR LOCATION: [Non-structural protein 4]: Host membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | PTM: Specific enzymatic cleavages in vivo by its own protease yield mature proteins. 3CL-PRO is autocatalytically processed (By similarity). {ECO:0000250}. |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 765,975 |
| Kinetics | |
| Metal Binding | METAL 5293; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5296; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5304; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5307; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5314; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5317; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5321; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5327; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5336; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5338; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5355; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 5359; /note=Zinc 3; /evidence=ECO:0000255|PROSITE-ProRule:PRU00986; METAL 6025; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6029; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6033; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298; METAL 6048; /note=Zinc 4; /evidence=ECO:0000255|PROSITE-ProRule:PRU01298 |
| Rhea ID | RHEA:21248; RHEA:13065 |
| Cross Reference Brenda |