| IED ID | IndEnz0002001933 |
| Enzyme Type ID | protease001933 |
| Protein Name |
Calpain-like protease 1 EC 3.4.22.- Calpain-7 Cysteine protease RIM13 Regulator of IME2 protein 13 |
| Gene Name | RIM13 CPL1 YMR154C YM8520.03C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MNDWHEFNAAIKSIYCNAEGDSSSIINRLVGLAMKSEDSTFIEAVLVLKENVSKVDKQLRFLWLTSTINSRFYPPIPISEASPVSWNKTEYCAPGTEELQRRYPGRAKLQNEEDYSGGIEQCRDVPDCSLVASLINLRSKNLNLPLIKQISSTKYHVNLSFNGSNKRLVTVDISQIPTSVDGKQLSLKSNDISDKIGELALLLVSKGTYSTDGSNISIDTYRLSGFLPEITQVNSYPFEKLWKFHKSNLCLMGAGTGNRSNDMIKPLVENHDYSIIDITYDSRLVKLRDPRNSALNVEISYEQYLKNFKQLYLNWNQEKLFKRSQVLHFRYDTSRYNKFSIVADKPLFHLVNNSKVTETVWLLLESHLQDEGSQENRSVSFLNEAPECIICPIEPPVECGGNHIGLQLVKLRLDAETERLLYCYSTTNNNFSIHSFSVVKEICFQRLKDTKSLFAKVLFSFPYEIEGKASFDTCNFFQNPTFELEVHSEQDYQVLMDAACISTSSHDLINIQVYYFNDYELIKPIMFDNHYQPGQGLKQDVPILTNVKYMIVCSTYGPPASTEFELLASIRLSSSWRLISGITLRSVNLIYGTYPYHCRNRFHWKETSDKLKIQMTLPTKKYSTNKLFIRVVPVESSARLRMRCNIFEPESALCVYECQEYRTCPSGGIVIPDLEVSRTNIVVLMIERSVPISSCLPTEGQLDELELFVGSSQKIRIEKYSDDVIPK |
| Enzyme Length | 727 |
| Uniprot Accession Number | Q03792 |
| Absorption | |
| Active Site | ACT_SITE 128; /evidence=ECO:0000250; ACT_SITE 271; /evidence=ECO:0000250; ACT_SITE 296; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth. Probably the protease that cleaves RIM101. {ECO:0000269|PubMed:11050096, ECO:0000269|PubMed:9017390, ECO:0000269|PubMed:9928935}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (1); Mutagenesis (1) |
| Keywords | Hydrolase;Protease;Reference proteome;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 11595789; 12208998; 15579677; 16554755; 17496126; 18287536; 18394190; 18467557; 18806212; 19536198; 19725809; 20842291; 21471218; 21908597; 22360598; 22615903; 22865500; 22918244; 22928083; 23656787; 23836928; 25002535; 25239548; 25460796; 25483081; 25859664; 25934176; 26721270; 8417990; |
| Motif | |
| Gene Encoded By | |
| Mass | 83,124 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |