Detail Information for IndEnz0002001933
IED ID IndEnz0002001933
Enzyme Type ID protease001933
Protein Name Calpain-like protease 1
EC 3.4.22.-
Calpain-7
Cysteine protease RIM13
Regulator of IME2 protein 13
Gene Name RIM13 CPL1 YMR154C YM8520.03C
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Enzyme Sequence MNDWHEFNAAIKSIYCNAEGDSSSIINRLVGLAMKSEDSTFIEAVLVLKENVSKVDKQLRFLWLTSTINSRFYPPIPISEASPVSWNKTEYCAPGTEELQRRYPGRAKLQNEEDYSGGIEQCRDVPDCSLVASLINLRSKNLNLPLIKQISSTKYHVNLSFNGSNKRLVTVDISQIPTSVDGKQLSLKSNDISDKIGELALLLVSKGTYSTDGSNISIDTYRLSGFLPEITQVNSYPFEKLWKFHKSNLCLMGAGTGNRSNDMIKPLVENHDYSIIDITYDSRLVKLRDPRNSALNVEISYEQYLKNFKQLYLNWNQEKLFKRSQVLHFRYDTSRYNKFSIVADKPLFHLVNNSKVTETVWLLLESHLQDEGSQENRSVSFLNEAPECIICPIEPPVECGGNHIGLQLVKLRLDAETERLLYCYSTTNNNFSIHSFSVVKEICFQRLKDTKSLFAKVLFSFPYEIEGKASFDTCNFFQNPTFELEVHSEQDYQVLMDAACISTSSHDLINIQVYYFNDYELIKPIMFDNHYQPGQGLKQDVPILTNVKYMIVCSTYGPPASTEFELLASIRLSSSWRLISGITLRSVNLIYGTYPYHCRNRFHWKETSDKLKIQMTLPTKKYSTNKLFIRVVPVESSARLRMRCNIFEPESALCVYECQEYRTCPSGGIVIPDLEVSRTNIVVLMIERSVPISSCLPTEGQLDELELFVGSSQKIRIEKYSDDVIPK
Enzyme Length 727
Uniprot Accession Number Q03792
Absorption
Active Site ACT_SITE 128; /evidence=ECO:0000250; ACT_SITE 271; /evidence=ECO:0000250; ACT_SITE 296; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.22.-
Enzyme Function FUNCTION: Required for the proteolytic cleavage of the transcriptional repressor RIM101 in response to alkaline ambient pH, which is necessary for sporulation and invasive growth. Probably the protease that cleaves RIM101. {ECO:0000269|PubMed:11050096, ECO:0000269|PubMed:9017390, ECO:0000269|PubMed:9928935}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Domain (1); Mutagenesis (1)
Keywords Hydrolase;Protease;Reference proteome;Thiol protease
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11595789; 12208998; 15579677; 16554755; 17496126; 18287536; 18394190; 18467557; 18806212; 19536198; 19725809; 20842291; 21471218; 21908597; 22360598; 22615903; 22865500; 22918244; 22928083; 23656787; 23836928; 25002535; 25239548; 25460796; 25483081; 25859664; 25934176; 26721270; 8417990;
Motif
Gene Encoded By
Mass 83,124
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda