Detail Information for IndEnz0002001957
IED ID IndEnz0002001957
Enzyme Type ID protease001957
Protein Name Single-stranded DNA-binding protein, mitochondrial
Mt-SSB
MtSSB
PWP1-interacting protein 17
Gene Name SSBP1 SSBP
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MFRRPVLQVLRQFVRHESETTTSLVLERSLNRVHLLGRVGQDPVLRQVEGKNPVTIFSLATNEMWRSGDSEVYQLGDVSQKTTWHRISVFRPGLRDVAYQYVKKGSRIYLEGKIDYGEYMDKNNVRRQATTIIADNIIFLSDQTKEKE
Enzyme Length 148
Uniprot Accession Number Q04837
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA) (PubMed:21953457, PubMed:23290262, PubMed:31550240). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork (PubMed:21953457, PubMed:12975372, PubMed:26446790, PubMed:15167897, PubMed:31550240). Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity (PubMed:26446790, PubMed:21953457, PubMed:31550240). In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK (PubMed:12975372). May also function in mtDNA repair (PubMed:23290262). {ECO:0000269|PubMed:12975372, ECO:0000269|PubMed:15167897, ECO:0000269|PubMed:21953457, ECO:0000269|PubMed:23290262, ECO:0000269|PubMed:26446790, ECO:0000269|PubMed:31550240}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Beta strand (9); Chain (1); Domain (1); Helix (1); Modified residue (4); Mutagenesis (5); Natural variant (7); Transit peptide (1)
Keywords 3D-structure;Acetylation;DNA replication;DNA-binding;Direct protein sequencing;Disease variant;Mitochondrion;Mitochondrion nucleoid;Phosphoprotein;Reference proteome;Transit peptide
Interact With O75603; P47929; Q7L8S5; Itself; P04618
Induction
Subcellular Location SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:18063578, ECO:0000269|PubMed:31550237, ECO:0000269|PubMed:31550240}. Mitochondrion matrix, mitochondrion nucleoid {ECO:0000269|PubMed:18063578, ECO:0000269|PubMed:31550240}.
Modified Residue MOD_RES 67; /note="Phosphoserine"; /evidence="ECO:0000250|UniProtKB:Q9CYR0"; MOD_RES 79; /note="Phosphoserine"; /evidence="ECO:0007744|PubMed:17525332, ECO:0007744|PubMed:23186163"; MOD_RES 113; /note="N6-acetyllysine"; /evidence="ECO:0007744|PubMed:19608861"; MOD_RES 122; /note="N6-succinyllysine"; /evidence="ECO:0000250|UniProtKB:Q9CYR0"
Post Translational Modification
Signal Peptide
Structure 3D X-ray crystallography (4)
Cross Reference PDB 1S3O; 2DUD; 3ULL; 6RUP;
Mapped Pubmed ID 12479417; 12577067; 15133161; 18305033; 18457437; 19066201; 19167051; 19738201; 19759019; 19786724; 19805454; 19951946; 20000738; 20186120; 20360068; 20434493; 20562859; 20877624; 21150319; 21242961; 21360678; 21851590; 21911578; 22153281; 22190034; 22575643; 22863774; 23184057; 23871696; 23902751; 23986477; 25036637; 25249620; 25609649; 25762445; 25852190; 26058080; 26170237; 26496610; 26638075; 27938330; 28210897; 28448822; 28486639; 28609781; 28778884; 29182774; 29522991; 29577982; 30256971; 30412255; 30415472; 30715486; 31998294; 33087282; 33671400; 33847964; 33847965; 33847968; 34548540; 34905022;
Motif
Gene Encoded By
Mass 17,260
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda