IED ID | IndEnz0002001989 |
Enzyme Type ID | protease001989 |
Protein Name |
Cyanophycinase EC 3.4.15.6 |
Gene Name | cphB slr2001 |
Organism | Synechocystis sp. (strain PCC 6803 / Kazusa) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Cyanobacteria/Melainabacteria group Cyanobacteria Synechococcales Merismopediaceae Synechocystis unclassified Synechocystis Synechocystis sp. PCC 6803 Synechocystis sp. (strain PCC 6803 / Kazusa) |
Enzyme Sequence | MPLSSQPAILIIGGAEDKVHGREILQTFWSRSGGNDAIIGIIPSASREPLLIGERYQTIFSDMGVKELKVLDIRDRAQGDDSGYRLFVEQCTGIFMTGGDQLRLCGLLADTPLMDRIRQRVHNGEISLAGTSAGAAVMGHHMIAGGSSGEWPNRALVDMAVGLGIVPEIVVDQHFHNRNRMARLLSAISTHPELLGLGIDEDTCAMFERDGSVKVIGQGTVSFVDARDMSYTNAALVGANAPLSLHNLRLNILVHGEVYHQVKQRAFPRVT |
Enzyme Length | 271 |
Uniprot Accession Number | P73832 |
Absorption | |
Active Site | ACT_SITE 132; /note=Charge relay system; /evidence=ECO:0000269|PubMed:19591842; ACT_SITE 174; /note=Charge relay system; /evidence=ECO:0000269|PubMed:19591842; ACT_SITE 201; /note=Charge relay system; /evidence=ECO:0000269|PubMed:19591842 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=[L-4-(L-arginin-2-N-yl)aspartate](n) + H2O = [L-4-(L-arginin-2-N-yl)aspartate](n-1) + L-4-(L-arginin-2-N-yl)aspartate; Xref=Rhea:RHEA:12845, Rhea:RHEA-COMP:13728, Rhea:RHEA-COMP:13734, ChEBI:CHEBI:15377, ChEBI:CHEBI:137986, ChEBI:CHEBI:137991; EC=3.4.15.6; Evidence={ECO:0000269|PubMed:10429200, ECO:0000269|PubMed:19591842}; |
DNA Binding | |
EC Number | 3.4.15.6 |
Enzyme Function | FUNCTION: Exopeptidase that catalyzes the hydrolytic cleavage of multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) into aspartate-arginine dipeptides. {ECO:0000269|PubMed:10429200, ECO:0000269|PubMed:19591842}. |
Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 35 degrees Celsius. {ECO:0000269|PubMed:10429200, ECO:0000269|PubMed:19591842}; |
PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:10429200, ECO:0000269|PubMed:19591842}; |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Beta strand (16); Chain (1); Helix (10); Initiator methionine (1); Mutagenesis (11); Turn (3) |
Keywords | 3D-structure;Direct protein sequencing;Hydrolase;Protease;Reference proteome;Serine protease |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | X-ray crystallography (1) |
Cross Reference PDB | 3EN0; |
Mapped Pubmed ID | 18000013; |
Motif | |
Gene Encoded By | |
Mass | 29,390 |
Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.2 uM for cyanophycin (at 30 degrees Celsius and pH 8.0) {ECO:0000269|PubMed:10429200, ECO:0000269|PubMed:19591842}; Note=kcat is 16.5 sec(-1) for cyanophycin.; |
Metal Binding | |
Rhea ID | RHEA:12845 |
Cross Reference Brenda | 3.4.15.6; |