| IED ID | IndEnz0002001993 |
| Enzyme Type ID | protease001993 |
| Protein Name |
Crustapain EC 3.4.22.- NsCys |
| Gene Name | Cys |
| Organism | Pandalus borealis (Northern red shrimp) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Crustacea Multicrustacea Malacostraca Eumalacostraca Eucarida Decapoda Pleocyemata Caridea Pandaloidea Pandalidae (pandalid shrimps) Pandalus Pandalus borealis (Northern red shrimp) |
| Enzyme Sequence | MRSLFLILLGLAAVSAIGEWENFKTKFGKKYANSEEESHRMSVFMDKLKFIQEHNERYDKGEVTYWLKINNFSDLTHEEVLATKTGMTRRRHPLSVLPKSAPTTPMAADVDWRNKGAVTPVKDQGQCGSCWAFSAVAALEGAHFLKTGDLVSLSEQNLVDCSSSYGNQGCNGGWPYQAYQYIIANRGIDTESSYPYKAIDDNCRYDAGNIGATVSSYVEPASGDESALQHAVQNEGPVSVCIDAGQSSFGSYGGGVYYEPNCDSWYANHAVTAVGYGTDANGGDYWIVKNSWGAWWGESGYIKMARNRDNNCAIATYSVYPVV |
| Enzyme Length | 323 |
| Uniprot Accession Number | Q86GF7 |
| Absorption | |
| Active Site | ACT_SITE 130; /evidence=ECO:0000250|UniProtKB:P07711; ACT_SITE 269; /evidence=ECO:0000250|UniProtKB:P07711; ACT_SITE 290; /evidence=ECO:0000250|UniProtKB:P07711 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Cysteine protease which may be involved in digestion. Shows highest affinity for proline at the P2 position. Cleaves valine more efficiently than leucine and exhibits 10-fold lower affinity for phenylalanine than proline. Can cleave type I collagen. {ECO:0000269|PubMed:12869537}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 40 degrees Celsius but displays considerable activity as low as 20 degrees Celsius. Thermostable. Retains 60% of initial activity after incubation at 50 degrees Celsius for 2 hours. {ECO:0000269|PubMed:12869537}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0. Unstable at pH values less than 5.0 and remains fully active over a wide alkaline pH range. {ECO:0000269|PubMed:12869537}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Disulfide bond (3); Propeptide (1); Signal peptide (1) |
| Keywords | Collagen degradation;Digestion;Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Secreted;Signal;Thiol protease;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..16; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 35,525 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=118 uM for Z-Phe-Arg-MCA (at pH 4.0) {ECO:0000269|PubMed:12869537}; KM=78 uM for Z-Phe-Arg-MCA (at pH 6.0) {ECO:0000269|PubMed:12869537}; KM=86 uM for Z-Phe-Arg-MCA (at pH 8.0) {ECO:0000269|PubMed:12869537}; KM=98 uM for Z Pro-Arg-MCA (at pH 4.0) {ECO:0000269|PubMed:12869537}; KM=65 uM for Z-Pro-Arg-MCA (at pH 6.0) {ECO:0000269|PubMed:12869537}; KM=70 uM for Z-Pro-Arg-MCA (at pH 8.0) {ECO:0000269|PubMed:12869537}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |