Detail Information for IndEnz0002002003
IED ID IndEnz0002002003
Enzyme Type ID protease002003
Protein Name Fibrinolytic enzyme
NJF
EC 3.4.21.-
Fragments
Gene Name
Organism Hediste japonica (Polychaete worm) (Neanthes japonica)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Spiralia Lophotrochozoa Annelida Polychaeta (polychaetes) Errantia Phyllodocida Nereididae (sandworms) Hediste Hediste japonica (Polychaete worm) (Neanthes japonica)
Enzyme Sequence NYLHDTVGRLWGPSRAGLLDGLDWMVGDVQSR
Enzyme Length 32
Uniprot Accession Number P86330
Absorption
Active Site
Activity Regulation ACTIVITY REGULATION: Inhibited by phenylmethanesulfonyl fluoride (PMSF). Not inhibited by EDTA, EGTA, beta-mercaptoethanol, indoacetamide, benzamidine, aprotinin, pepstatin A and trypsin inhibitor. {ECO:0000269|PubMed:19896371}.
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.21.-
Enzyme Function FUNCTION: Plasmin-like serine protease. Has fibrinolytic and fibrinogenolytic but not plasminogenolytic activity. Cleaves after Arg and Lys residues. {ECO:0000269|PubMed:19896371}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 degrees Celsius. Activity is stable from 40 to 80 degrees Celsius. {ECO:0000269|PubMed:19896371};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 9.0. Activity is stable from pH 6 to 11 but decreases sharply below pH 6. {ECO:0000269|PubMed:19896371};
Pathway
nucleotide Binding
Features Chain (1); Non-adjacent residues (2); Non-terminal residue (2)
Keywords Blood coagulation;Direct protein sequencing;Fibrinogenolytic toxin;Fibrinolysis;Hemostasis;Hemostasis impairing toxin;Hydrolase;Protease;Serine protease;Toxin
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification PTM: The N-terminus is blocked. {ECO:0000269|PubMed:19896371}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 3,585
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda