IED ID | IndEnz0002002008 |
Enzyme Type ID | protease002008 |
Protein Name |
Hirudin-2 Hirudin II |
Gene Name | |
Organism | Hirudo medicinalis (Medicinal leech) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Spiralia Lophotrochozoa Annelida Clitellata Hirudinea (leeches) Hirudinida Hirudiniformes Hirudinidae Hirudo Hirudo medicinalis (Medicinal leech) |
Enzyme Sequence | ITYTDCTESGQDLCLCEGSNVCGKGNKCILGSNGEENQCVTGEGTPKPQSHNDGDFEEIPEEYLQ |
Enzyme Length | 65 |
Uniprot Accession Number | P28504 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | |
DNA Binding | |
EC Number | |
Enzyme Function | FUNCTION: Hirudin is a potent thrombin-specific protease inhibitor. It forms a stable non-covalent complex with alpha-thrombin, thereby abolishing its ability to cleave fibrinogen. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Chain (1); Disulfide bond (3); Glycosylation (1); Helix (1); Modified residue (1); Region (3) |
Keywords | 3D-structure;Direct protein sequencing;Disulfide bond;Glycoprotein;Protease inhibitor;Secreted;Serine protease inhibitor;Sulfation |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
Modified Residue | MOD_RES 63; /note=Sulfotyrosine; /evidence=ECO:0000250 |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | X-ray crystallography (51) |
Cross Reference PDB | 1A3B; 1A3E; 1ABI; 1C1U; 1C1V; 1C1W; 1C4U; 1C5L; 1C5N; 1C5O; 1D9I; 1FPC; 1GHV; 1GHW; 1GHX; 1GHY; 1GJ4; 1GJ5; 1IHS; 1NM6; 1NT1; 1NY2; 1O2G; 1QBV; 1SB1; 1SL3; 1TA2; 1TA6; 1TMT; 1TWX; 1VR1; 1XM1; 1YPE; 1YPG; 1YPJ; 1YPK; 1YPL; 1YPM; 1ZRB; 2GDE; 2HGT; 2PKS; 3VXE; 3VXF; 6ZUG; 6ZUH; 6ZUN; 6ZUU; 6ZUW; 6ZUX; 6ZV7; 6ZV8; |
Mapped Pubmed ID | 10543954; 10713516; 10779411; 11292354; 11731301; 12598231; 12742021; 12873514; 1445905; 15051174; 15163182; 15299843; 15572256; 15801822; 16374786; 16895415; 17341023; 18019535; 1942057; 23712263; 33108181; 8251938; 8272424; 9468142; 9622548; 9703466; 9772168; |
Motif | |
Gene Encoded By | |
Mass | 6,987 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |