| IED ID | IndEnz0002002045 |
| Enzyme Type ID | protease002045 |
| Protein Name |
50 kDa hatching enzyme HE HEZ EC 3.4.24.12 Envelysin Sea-urchin-hatching proteinase Cleaved into: 38 kDa hatching enzyme; 32 kDa hatching enzyme non-specific; 15 kDa peptide |
| Gene Name | |
| Organism | Hemicentrotus pulcherrimus (Sea urchin) (Strongylocentrotus pulcherrimus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Echinodermata Eleutherozoa Echinozoa Echinoidea (sea urchins) Euechinoidea Echinacea Camarodonta Echinidea Strongylocentrotidae Hemicentrotus Hemicentrotus pulcherrimus (Sea urchin) (Strongylocentrotus pulcherrimus) |
| Enzyme Sequence | MANFCLIFAAVFLTRLTTVLNTPISVTFGPTQLTDITKLVSETGDDFGLTTPRSAILTTVSEDDSDDDDGGESVEDTTILQTTTSSEIVISGIVVDEDIDESKVVKLKANLEQFGYVPLGSTFGEANINYTSAILEYQQNGGINQTGILDAETAALLDTPRCGVPDILPYVTGGIAWPRNVAVTYSFGTLSNDLSQTAIKNELRRAFQVWDDVSSLTFREVVDSSSVDIRIKFGSYEHGDGISFDGQGGVLAHAFLPRNGDAHFDDSERWTIGTNSGTNLFQVAAHEFGHSLGLYHSDVQSALMYPYYRGYNPNFNLDRDDIAGITSLYGRNTGSTTTTTRRPTITRTTTRRTTTRRTTTQLATTQTTTIRPPTYPTPPRQACTGSFDAVIKDNSDRIYALAGRYYWRLDQASPSWGVVRNRFGFDLPENVDASFQNGIFSYFFSGCYYYYQTSTRRRFPRTPFNRRWVGLPCDIDAVYKSGDSGTTYFFKGRFVYKFSSSNQLQRRSPISSYFRNTPYALRDGVEAVVRVDDVYLHFYRDGRYYRMIESTKQFVNFPNGLSYRDVIDTLIPQCRSLNLSVEIESCSNSSE |
| Enzyme Length | 591 |
| Uniprot Accession Number | P91953 |
| Absorption | |
| Active Site | ACT_SITE 287; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Hydrolysis of proteins of the fertilization envelope and dimethylcasein.; EC=3.4.24.12; |
| DNA Binding | |
| EC Number | 3.4.24.12 |
| Enzyme Function | FUNCTION: Allows the sea urchin to digest the protective envelope derived from the egg extracellular matrix; thus allowing the sea urchin to swim freely. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (4); Compositional bias (1); Disulfide bond (1); Glycosylation (4); Metal binding (4); Motif (1); Propeptide (1); Region (1); Repeat (4); Sequence conflict (9); Signal peptide (1); Site (2) |
| Keywords | Autocatalytic cleavage;Calcium;Direct protein sequencing;Disulfide bond;Glycoprotein;Hydrolase;Metal-binding;Metalloprotease;Protease;Repeat;Signal;Zinc;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000305 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 160..167; /note=Cysteine switch; /evidence=ECO:0000250 |
| Gene Encoded By | |
| Mass | 66,126 |
| Kinetics | |
| Metal Binding | METAL 162; /note=Zinc; in inhibited form; /evidence=ECO:0000250; METAL 286; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 290; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 296; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
| Rhea ID | |
| Cross Reference Brenda | 3.4.24.12; |