IED ID | IndEnz0002002142 |
Enzyme Type ID | protease002142 |
Protein Name |
Leukotriene A-4 hydrolase homolog LTA-4 hydrolase EC 3.3.2.6 Leukotriene A 4 hydrolase |
Gene Name | BC1G_09514 |
Organism | Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Leotiomycetes Helotiales Sclerotiniaceae Botrytis Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea) Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) |
Enzyme Sequence | MATVSTINMPRDPNTLSNYNNWRTKHTIADLAIDFTKQRVHGTVTLQLESITDKESEEIILDTSFVDVQSVAVDGNKTGEWTLEKRIEPFGTPLSVKIPGGAAKGTVIALAITLSTTDKCTALQWLTPAQTSNKKFPYMFSQCQAIHNRSIFPCQDTPDVKSTYDFRIRSPLPVLASGLPRGAGSFVHGENGESGTLLYSFYQEIPMPSYLFALASGDIATASIGPRSLVSTGPEELIGAKWELERDTEKFIETIEKIVYPYEWTQYNVLVLPPSFPYGGMENPVFTFATPTIISGDRENVDVVAHELAHSWSGNLVSNASWEHFWLNEGWTVYLERRIIAAVHGEAYRDFSSIIGWKALEDSVKLYGEDHEFTKLIVDLKGKDPDDAFSSVPYEKGFHFLYYLERLVGKPSWDKFIPHYFTTWKKKSLDSYDFKATLLDFFASDSAASKALESVDWDSWFYKPGLPSKPEFDTSLVDKCYALAKKWESKDYTPSPSDIEGWAANQVVVFLQQVQLFTTPLTPVQSQAMGKAYNLVNTKNVELSSRYFGVGLAAKDETVYQPTAELLGKVGRMKFVRTLYRKLVVVDRKLAVETFEKNKDFYHPICRDQVEKDLKE |
Enzyme Length | 616 |
Uniprot Accession Number | A6SAG8 |
Absorption | |
Active Site | ACT_SITE 307; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; ACT_SITE 394; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + leukotriene A4 = leukotriene B4; Xref=Rhea:RHEA:22324, ChEBI:CHEBI:15377, ChEBI:CHEBI:57461, ChEBI:CHEBI:57463; EC=3.3.2.6; |
DNA Binding | |
EC Number | 3.3.2.6 |
Enzyme Function | FUNCTION: Aminopeptidase that preferentially cleaves tripeptides. Also has low epoxide hydrolase activity (in vitro). Can hydrolyze an epoxide moiety of LTA(4) to form LTB(4) (in vitro) (By similarity). {ECO:0000250}. |
Temperature Dependency | |
PH Dependency | |
Pathway | PATHWAY: Lipid metabolism; leukotriene B4 biosynthesis. |
nucleotide Binding | |
Features | Active site (2); Chain (1); Erroneous initiation (1); Metal binding (3); Region (2) |
Keywords | Cytoplasm;Hydrolase;Leukotriene biosynthesis;Metal-binding;Metalloprotease;Nucleus;Protease;Zinc |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 69,504 |
Kinetics | |
Metal Binding | METAL 306; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 310; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095; METAL 329; /note=Zinc; catalytic; /evidence=ECO:0000255|PROSITE-ProRule:PRU10095 |
Rhea ID | RHEA:22324 |
Cross Reference Brenda |