| IED ID | IndEnz0002002262 |
| Enzyme Type ID | protease002262 |
| Protein Name |
RNA-directed RNA polymerase L Protein L EC 2.7.7.48 EC 3.1.-.- Large structural protein Replicase Transcriptase |
| Gene Name | L |
| Organism | Bunyamwera virus (BUNV) |
| Taxonomic Lineage | Viruses Riboviria Orthornavirae Negarnaviricota Polyploviricotina Ellioviricetes Bunyavirales Peribunyaviridae Orthobunyavirus Bunyamwera orthobunyavirus Bunyamwera virus (BUNV) |
| Enzyme Sequence | MEDQAYDQYLHRIQAARTATVAKDISADILEARHDYFGRELCNSLGIEYKNNVLLDEIILDVVPGVNLLNYNIPNVTPDNYIWDGHFLIILDYKVSVGNDSSEITYKKYTSLILPVMSELGIDTEIAIIRANPVTYQISIIGEEFKQRFPNIPIQLDFGRFFELRKMLLDKFADDEEFLMMIAHGDFTLTAPWCTSDTPELEEHEIFQEFINSMPPRFVSLFKEAVNFSAYSSERWNTFLYRARAETEVDYNQFLSDKAHKIFMLEGDYMRPTQAEIDKGWELMSQRVYTEREIITDVTKQKPSIHFIWVKNADRKLIGSTAKLIYLSNSLQSITEQSTWTDALKAIGKSMDIDGKVGQYETLCAERKMIARSTGKKVDNKRLEAVKIGNALVLWEQQFILANDLFKNQERQKFMKNFFGIGKHKSFKDKTSSDIETDKPKILDFNNTIVLMAARTMVNKNKALLAKDNTLQDLHPIIMQYASEIKEASKDTFDALLKISKTCFWQCIVDVSTIMRNILAVSQYNRHNTFRVAMCANDSVYALVFPSSDIKTKRATVVFSIVCMHKEKNDLMDAGALFTTLECKNKEYISISKAIRLDKERCQRIVSSPGLFILSSMLLYNNNPEVNLVDVLNFTFYTSLSITKSMLSLTEPSRYMIMNSLAISSHVRDYIAEKFSPYTKTLFSVYMVNLIKRGCASANEQSSKIQLRNIYLSDYDITQKGVNDGRNLDSIWFPGKVNLKEYINQIYLPFYFNAKGLHEKHHVMIDLAKTVLEIEMNQRSDNLGIWSKAEKKQHVNLPILIHSIAKSLILDTSRHNHLRNRVESRNNFRRSITTISTFTSSKSCIKIGDFREIKDKETEKSKKSTEKFDKKFRLSNPLFLEDEEANLEVQHCNYRALIQKIPNYKDYISVKVFDRLYELLKNGVLTDKPFIELAMEMMKNHKEFSFTFFNKGQKTAKDREIFVGEFEAKMCMYVVERISKERCKLNTDEMISEPGDSKLKILEKKAEEEIRYIVERTKDSIIKGDPSKALKLEINADMSKWSAQDVFYKYFWLIAMDPILYPAEKTRILYFMCNYMQKLLILPDDLIANILDQKRPYNDDLILEMTNGLNYNYVQIKRNWLQGNFNYISSYVHSCAMLVYKDILKECMKLLDGDCLINSMVHSDDNQTSLAIIQNKVSDQIVIQYAANTFESVCLTFGCQANMKKTYITHTCKEFVSLFNLHGEPLSVFGRFLLPSVGDCAYIGPYEDLASRLSAAQQSLKHGCPPSLVWLAISCSHWITFFTYNMLDDQINAPQQHLPFNNRKEIPVELNGYLNAPLYLIALVGLEAGNLWFLINILKKLVPLDKQKETIQSQCLHLCNSIDKLTESEKFKLKILRYLTLDTEMSVDNNMGETSDMRSRSLLTPRKFTTLGSLNKLVSYNDFRSSLDDQRFTDNLNFMLNNPELLVTKGENKEQFMQSVLFRYNSKRFKESLSIQNPAQLFIEQILFSHKPIIDYSSIFDKLTSLAEADIIEELPEIIGRVTFPQAYQMINRDIGQLPLDIDDIKLIFRYCILNDPLMITAANTSLLCVKGTPQDRTGLSASQMPEFRNMKLIHHSPALVLKAFSKGTSDIPGADPIELEKDLHHLNEFVETTAIKEKILHNIDNPPKHLIGNEILIYRIREMTKLYQVCYDYVKSTEHKVKIFILPMKSYTAIDFCTLIQGNTISDNKWYTMHYLKQIASGSIKGNIVTTSTSEQIIANECFRVLCHFADSFVEEASRLSFINEVLDNFTYKNISVNSLFNTLLASTTRLDFIPLLFRLKVLTQTDLNRFDALKTNERVSWNNWQTNRSLNSGLIDLTISGYLRSIRVVGEDNKLKIAELTIPNFYPNTVFHAGNKLLNSRHGLKFEYMEEIVLDEKYNYYITYQKKRAHIYTYQVSTIEHILRRNNEGLQSRGPRYNKMVPVCPVVLSVRDELFRMSLENVFSLNMTNFSMSRLFVSPDEVATVKKAHMSKMMFFSGPTIKAGIINLTSLMRTQELLTLNYDNLCKSSIVPFCRILECNGDEQGELIFLSDEVMDFTISEEIESMPLFTIRYQKRGTEIMTYKNAIMKLVSAGVDEIKEVFDFSKQGFYSKKNLGIINTICSIINILETNEWSTILYNSFHIAMLLESMDREFHMFTLPEAFFINVAGGVVNWTKLLKFIKSLPVIEQEPWSMMMSRFVEKTVYLIEREMNKDVDFTDFLDELEFSSGKSLFTFF |
| Enzyme Length | 2238 |
| Uniprot Accession Number | P20470 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539}; |
| DNA Binding | |
| EC Number | 2.7.7.48; 3.1.-.- |
| Enzyme Function | FUNCTION: RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism. It consists in the hijacking and cleavage via N-terminal endonuclease activity of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are not polyadenylated. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. {ECO:0000269|PubMed:12642095, ECO:0000269|PubMed:19141438}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Chain (1); Domain (1); Metal binding (5) |
| Keywords | Host cytoplasm;Hydrolase;Manganese;Metal-binding;Nucleotide-binding;Nucleotidyltransferase;Protease;RNA-directed RNA polymerase;Reference proteome;Thiol protease;Transferase;Ubl conjugation pathway;Viral RNA replication;Virion |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:19141438}. Virion {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 258,670 |
| Kinetics | |
| Metal Binding | METAL 34; /note=Manganese 1; via tele nitrogen; /evidence=ECO:0000250|UniProtKB:A5HC98; METAL 79; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:A5HC98; METAL 79; /note=Manganese 2; /evidence=ECO:0000250|UniProtKB:A5HC98; METAL 92; /note=Manganese 1; /evidence=ECO:0000250|UniProtKB:A5HC98; METAL 93; /note=Manganese 1; via carbonyl oxygen; /evidence=ECO:0000250|UniProtKB:A5HC98 |
| Rhea ID | RHEA:21248 |
| Cross Reference Brenda |