Detail Information for IndEnz0002002273
IED ID IndEnz0002002273
Enzyme Type ID protease002273
Protein Name Methionine aminopeptidase 1D, mitochondrial
MAP 1D
MetAP 1D
EC 3.4.11.18
Methionyl aminopeptidase type 1D, mitochondrial
Peptidase M 1D
Gene Name metap1d map1d zgc:110461
Organism Danio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Actinopterygii Actinopteri Neopterygii Teleostei Osteoglossocephalai Clupeocephala Otomorpha Ostariophysi Otophysi Cypriniphysae Cypriniformes (carps and others) Cyprinoidei Danionidae Danioninae Danio Danio rerio (Zebrafish) (Brachydanio rerio)
Enzyme Sequence MAAPCAAQCLYRTGGLRLLQRISRLPHCHKDASLAHQCQFHRSFFWRKPKTSHSVVRPAIVRPAYPVPKHIQRPDYVSSSKVPEWPDYIEIKDEEQIQGLRRACQLARHILLLTGNSLKVGMTTDEIDFIVHQEAIRHNGYPSPLHYGGFPKSVCTSVNNVVCHGIPDSRPLQDGDIINIDVTVYLEGYHGDTSETFLIGSVNDQGRKLVDVARQCRDQAIAACGPGQPLCVIGNIISNIANSNGFRVCPYFIGHGIGEYFHGHPEIWHHANDNDLKMEEGMSFTIEPILMEGTSGFRILSDKWTAVSVDDKRSAQFEHTVVITSDGVEILTKLPEED
Enzyme Length 338
Uniprot Accession Number Q4VBS4
Absorption
Active Site
Activity Regulation
Binding Site BINDING 164; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; BINDING 262; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_03174
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.; EC=3.4.11.18; Evidence={ECO:0000255|HAMAP-Rule:MF_03174};
DNA Binding
EC Number 3.4.11.18
Enzyme Function FUNCTION: Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed. {ECO:0000255|HAMAP-Rule:MF_03174}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Binding site (2); Chain (1); Metal binding (7); Transit peptide (1)
Keywords Aminopeptidase;Hydrolase;Metal-binding;Mitochondrion;Protease;Reference proteome;Transit peptide
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03174}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 37,785
Kinetics
Metal Binding METAL 181; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 192; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 192; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 255; /note=Divalent metal cation 2; catalytic; via tele nitrogen; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 287; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 318; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 318; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03174
Rhea ID
Cross Reference Brenda