| IED ID | IndEnz0002002274 |
| Enzyme Type ID | protease002274 |
| Protein Name |
Methionine aminopeptidase 1D, mitochondrial MAP 1D MetAP 1D EC 3.4.11.18 Methionyl aminopeptidase type 1D, mitochondrial Peptidase M 1D |
| Gene Name | metap1d map1d DDB_G0280127 |
| Organism | Dictyostelium discoideum (Slime mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Amoebozoa Evosea Eumycetozoa Dictyostelia (dictyostelid cellular slime molds) Dictyosteliales Dictyosteliaceae Dictyostelium Dictyostelium discoideum (Slime mold) |
| Enzyme Sequence | MNKILKNIINKSSINNVFKTSFNGGISSSSSSSSSYLNNNNNIIKSYNVQQKQQQRYYSSFEDDLSPKKLKEKILENETEEIRDFVRSQRLTKKTASPLEGMNRKERRKMTTKLYRNPDNLIRGGIVSPQPLIPAHIKKPKYVLGEPVIDFEIDDPIEIHTAESIEHMRVVGKMAKEVLEYAGTLVRPGITTDEIDKLVHQNIIDRGAYPSPLGYKGFPKSICTSINEVLCHGIPDDRPLEFGDIVKIDVTLYYNGYHGDTCATFPVGEIDSSSKRLIEATEKALYAAIGEVKDGALFNKIGKKIQLVANKYSLSVTPEFTGHGIGQLFHTAPFVFQCANEFDSVMKEGMIFTIEPVLVESTSPYAEWKMWDDKWTVSSREGGWSAQFEHTILVTKDGYEILTK |
| Enzyme Length | 404 |
| Uniprot Accession Number | Q54VU7 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 232; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; BINDING 330; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_03174 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.; EC=3.4.11.18; Evidence={ECO:0000255|HAMAP-Rule:MF_03174}; |
| DNA Binding | |
| EC Number | 3.4.11.18 |
| Enzyme Function | FUNCTION: Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). {ECO:0000255|HAMAP-Rule:MF_03174}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Binding site (2); Chain (1); Metal binding (7); Region (1); Transit peptide (1) |
| Keywords | Aminopeptidase;Hydrolase;Metal-binding;Mitochondrion;Protease;Reference proteome;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03174}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 45,549 |
| Kinetics | |
| Metal Binding | METAL 249; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 260; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 260; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 323; /note=Divalent metal cation 2; catalytic; via tele nitrogen; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 355; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 389; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03174; METAL 389; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03174 |
| Rhea ID | |
| Cross Reference Brenda |