Detail Information for IndEnz0002002361
IED ID IndEnz0002002361
Enzyme Type ID protease002361
Protein Name Methionine aminopeptidase 2-1
MAP 2-1
MetAP 2-1
EC 3.4.11.18
Peptidase M
Gene Name BDBG_06837
Organism Blastomyces gilchristii (strain SLH14081) (Blastomyces dermatitidis)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Onygenales Ajellomycetaceae Blastomyces Blastomyces gilchristii Blastomyces gilchristii (strain SLH14081) (Blastomyces dermatitidis)
Enzyme Sequence MAAQVTPELANLNSKPEGGAPPKQVPVKDVPENEDVESDDDNEGDQGAGEPGSTGAAKKKKKKKPKKKKKGGPKVQTEPPRVILSSIFPNNDYPVGELVEYKDDNAYRTTNEEKRYLDRMNNDFLSEYRYAAEVHRQVRQYAQKTIKPGQTLTEIAEGIEDSVRALTGHDGLTEGDNLLGGIAFPTGVNLNHCAAHYSPNAGNKMVLQYEDVMKVDFGVHINGRIVDSAFTVAFDPVYDNLLAAVKDATNTGIREAGIDVRMSDIGAAIQETMESYEVEIKGTTYPVKPIRNLNGHTIGQFEIHGGKNGKSVPIVKGGDQSKMEEGEVYAIETFGSTGRGYVRDDMETSHYAKVPDAPNVPLRLSSAKNLLNVITKNFGTLPFCRRYLDRLGQDKYLLGLNNLVANGIVDAYPPLCDIKGSYTAQFEHTILLRPNIKEVISRGYDY
Enzyme Length 446
Uniprot Accession Number C5JW60
Absorption
Active Site
Activity Regulation
Binding Site BINDING 196; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; BINDING 304; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_03175
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.; EC=3.4.11.18; Evidence={ECO:0000255|HAMAP-Rule:MF_03175};
DNA Binding
EC Number 3.4.11.18
Enzyme Function FUNCTION: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). {ECO:0000255|HAMAP-Rule:MF_03175}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Binding site (2); Chain (1); Compositional bias (1); Metal binding (7); Region (1)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03175}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 49,025
Kinetics
Metal Binding METAL 216; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 227; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 227; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 296; /note=Divalent metal cation 2; catalytic; via tele nitrogen; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 332; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 427; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 427; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03175
Rhea ID
Cross Reference Brenda