Detail Information for IndEnz0002002375
IED ID IndEnz0002002375
Enzyme Type ID protease002375
Protein Name Methionine aminopeptidase 2B
MAP 2B
MetAP 2B
EC 3.4.11.18
Peptidase M
Gene Name MAP2B At3g59990 F24G16.26
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MASESPDVAVVAPVVENGGAESSNGKEEQLESELSKKLEIAEDGQEENDGEEGSKAETSTKKKKKKNKSKKKKELPQQTDPPSIPVVELFPSGEFPEGEIQEYKDDNLWRTTSEEKRELERFEKPIYNSVRRAAEVHRQVRKYVRSIVKPGMLMTDICETLENTVRKLISENGLQAGIAFPTGCSLNWVAAHWTPNSGDKTVLQYDDVMKLDFGTHIDGHIIDCAFTVAFNPMFDPLLAASREATYTGIKEAGIDVRLCDIGAAIQEVMESYEVEINGKVFQVKSIRNLNGHSIGPYQIHAGKSVPIVKGGEQTKMEEGEFYAIETFGSTGKGYVREDLECSHYMKNFDAGHVPLRLPRAKQLLATINKNFSTLAFCRRYLDRIGETKYLMALKNLCDSGIVQPYPPLCDVKGSYVSQFEHTILLRPTCKEVLSKGDDY
Enzyme Length 439
Uniprot Accession Number Q56Y85
Absorption
Active Site
Activity Regulation
Binding Site BINDING 192; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; BINDING 300; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_03175
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.; EC=3.4.11.18; Evidence={ECO:0000255|HAMAP-Rule:MF_03175};
DNA Binding
EC Number 3.4.11.18
Enzyme Function FUNCTION: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). {ECO:0000255|HAMAP-Rule:MF_03175}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Binding site (2); Chain (1); Compositional bias (1); Erroneous gene model prediction (1); Erroneous initiation (1); Metal binding (7); Region (1); Sequence conflict (2)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03175, ECO:0000269|PubMed:11060042}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 12185496; 15489341; 15681659;
Motif
Gene Encoded By
Mass 49,060
Kinetics
Metal Binding METAL 212; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 223; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 223; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 292; /note=Divalent metal cation 2; catalytic; via tele nitrogen; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 325; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 420; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 420; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03175
Rhea ID
Cross Reference Brenda