Detail Information for IndEnz0002002377
IED ID IndEnz0002002377
Enzyme Type ID protease002377
Protein Name E3 ubiquitin-protein ligase MARCHF6
EC 2.3.2.27
Doa10 homolog
Membrane-associated RING finger protein 6
Membrane-associated RING-CH protein VI
MARCH-VI
Protein TEB-4
RING finger protein 176
RING-type E3 ubiquitin transferase MARCHF6
Gene Name MARCHF6 KIAA0597 MARCH6 RNF176 TEB4
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPAGGNGAENVAADQPANPPAENAVVGENPDAQDDQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSVLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVMVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHYIVRKLAAPVISVLLLSLCVPYVIASGVVPLLGVTAEMQNLVHRRIYPFLLMVVVLMAILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGSSPPPPQSSQE
Enzyme Length 910
Uniprot Accession Number O60337
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:15673284};
DNA Binding
EC Number 2.3.2.27
Enzyme Function FUNCTION: E3 ubiquitin-protein ligase that promotes 'Lys-48'-linked ubiquitination of target proteins, leading to their proteasomal degradation (PubMed:15673284). Promotes ubiquitination of DIO2, leading to its degradation (PubMed:19651899). Promotes ubiquitination of SQLE, leading to its degradation (PubMed:24449766). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1 (PubMed:15673284). {ECO:0000269|PubMed:15673284, ECO:0000269|PubMed:19651899, ECO:0000269|PubMed:24449766}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Protein modification; protein ubiquitination. {ECO:0000269|PubMed:15673284}.
nucleotide Binding
Features Alternative sequence (2); Chain (1); Compositional bias (1); Erroneous initiation (2); Frameshift (1); Metal binding (8); Modified residue (1); Mutagenesis (1); Natural variant (1); Region (1); Sequence conflict (6); Topological domain (15); Transmembrane (14); Zinc finger (1)
Keywords Acetylation;Alternative splicing;Endoplasmic reticulum;Epilepsy;Membrane;Metal-binding;Reference proteome;Transferase;Transmembrane;Transmembrane helix;Ubl conjugation;Ubl conjugation pathway;Zinc;Zinc-finger
Interact With Q9UHX3; P41220-1
Induction
Subcellular Location SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:15673284}; Multi-pass membrane protein {ECO:0000269|PubMed:15673284}.
Modified Residue MOD_RES 1; /note=N-acetylmethionine; /evidence=ECO:0007744|PubMed:22814378
Post Translational Modification PTM: Auto-ubiquitinated, which results in proteasomal degradation. {ECO:0000269|PubMed:15673284}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 18264092; 18417469; 19084021; 20195357; 23232094; 24811749; 24840124; 26496610; 26514267; 26527619; 27068744; 29519897; 30202070; 30545937; 30658189; 31422115; 31904814; 32755570; 33049405; 34273954;
Motif
Gene Encoded By
Mass 102,545
Kinetics
Metal Binding METAL 9; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00623; METAL 12; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00623; METAL 26; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00623; METAL 28; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00623; METAL 36; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00623; METAL 39; /note=Zinc 1; /evidence=ECO:0000255|PROSITE-ProRule:PRU00623; METAL 52; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00623; METAL 55; /note=Zinc 2; /evidence=ECO:0000255|PROSITE-ProRule:PRU00623
Rhea ID
Cross Reference Brenda 2.3.2.27;