Detail Information for IndEnz0002002384
IED ID IndEnz0002002384
Enzyme Type ID protease002384
Protein Name Methionine aminopeptidase 2-2
MAP 2-2
MetAP 2-2
EC 3.4.11.18
Peptidase M
Gene Name An04g01330
Organism Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus niger Aspergillus niger (strain CBS 513.88 / FGSC A1513)
Enzyme Sequence MAAQASEKLQKLDLNGQSGDAEADAPAAGQTQAGEAEDDSDDDEVEDGNAAGEGAASGGGAKVQSSPPRVPLSTLFAGKEYPEGEIVEYQNENSYRTTNEEKRYLDRMKNDFLQEYRQAAEVHRQVRQYAQKTIKPGQTLTEIAEGIEESVRALTGHQGLEEGDNLKGGMGFPCGLSINHCAAHYTPNAGNKMVLQQGDVMKVDFGAHINGRIVDSAFTVAFDPVYDPLLAAVKDATNTGIREAGIDVRMSDIGAAIQEAMESYEVEINGTMYPVKCIRNLNGHNIDQHIIHGGKSVPIVKGGDQTKMEEGEVFAIETFGSTGKGYVREDMETSHYALIPDHSQVPLRLSSAKNLLNVINKNFGTLPFCRRYLDRLGQDKYLLGLNNLVSSGIVQDYPPLCDIKGSYTAQYEHTIVLRPNVKEVISRGDDY
Enzyme Length 431
Uniprot Accession Number A2QHX0
Absorption
Active Site
Activity Regulation
Binding Site BINDING 184; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; BINDING 292; /note=Substrate; /evidence=ECO:0000255|HAMAP-Rule:MF_03175
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.; EC=3.4.11.18; Evidence={ECO:0000255|HAMAP-Rule:MF_03175};
DNA Binding
EC Number 3.4.11.18
Enzyme Function FUNCTION: Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). {ECO:0000255|HAMAP-Rule:MF_03175}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Binding site (2); Chain (1); Compositional bias (1); Metal binding (7); Region (1)
Keywords Aminopeptidase;Cytoplasm;Hydrolase;Metal-binding;Protease;Reference proteome
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03175}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 46,964
Kinetics
Metal Binding METAL 204; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 215; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 215; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 284; /note=Divalent metal cation 2; catalytic; via tele nitrogen; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 317; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 412; /note=Divalent metal cation 1; /evidence=ECO:0000255|HAMAP-Rule:MF_03175; METAL 412; /note=Divalent metal cation 2; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_03175
Rhea ID
Cross Reference Brenda