Detail Information for IndEnz0002002440
IED ID IndEnz0002002440
Enzyme Type ID protease002440
Protein Name Glutamyl endopeptidase
EC 3.4.21.19
Glutamate-specific endopeptidase
GSE
Gene Name blaSE mpr BLi00340 BL01804
Organism Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus licheniformis Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46)
Enzyme Sequence MVSKKSVKRGLITGLIGISIYSLGMHPAQAAPSPHTPVSSDPSYKAETSVTYDPNIKSDQYGLYSKAFTGTGKVNETKEKAEKKSPAKAPYSIKSVIGSDDRTRVTNTTAYPYRAIVHISSSIGSCTGWMIGPKTVATAGHCIYDTSSGSFAGTATVSPGRNGTSYPYGSVKSTRYFIPSGWRSGNTNYDYGAIELSEPIGNTVGYFGYSYTTSSLVGTTVTISGYPGDKTAGTQWQHSGPIAISETYKLQYAMDTYGGQSGSPVFEQSSSRTNCSGPCSLAVHTNGVYGGSSYNRGTRITKEVFDNLTNWKNSAQ
Enzyme Length 316
Uniprot Accession Number P80057
Absorption
Active Site ACT_SITE 141; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10083; ACT_SITE 261; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10083
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa.; EC=3.4.21.19; Evidence={ECO:0000255|PROSITE-ProRule:PRU10083};
DNA Binding
EC Number 3.4.21.19
Enzyme Function FUNCTION: Specific for hydrolysis of peptide bonds on the carboxyl side of acidic amino acid residues, with a strong preference for Glu.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (2); Propeptide (1); Signal peptide (1)
Keywords Direct protein sequencing;Disulfide bond;Hydrolase;Protease;Reference proteome;Secreted;Serine protease;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..30; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 33,611
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda