Detail Information for IndEnz0002002444
IED ID IndEnz0002002444
Enzyme Type ID protease002444
Protein Name Granzyme M
EC 3.4.21.-
Met-1 serine protease
Hu-Met-1
Met-ase
Natural killer cell granular protease
Gene Name GZMM MET1
Organism Homo sapiens (Human)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Primates Haplorrhini Simiiformes Catarrhini Hominoidea (apes) Hominidae (great apes) Homininae Homo Homo sapiens (Human)
Enzyme Sequence MEACVSSLLVLALGALSVGSSFGTQIIGGREVIPHSRPYMASLQRNGSHLCGGVLVHPKWVLTAAHCLAQRMAQLRLVLGLHTLDSPGLTFHIKAAIQHPRYKPVPALENDLALLQLDGKVKPSRTIRPLALPSKRQVVAAGTRCSMAGWGLTHQGGRLSRVLRELDLQVLDTRMCNNSRFWNGSLSPSMVCLAADSKDQAPCKGDSGGPLVCGKGRVLARVLSFSSRVCTDIFKPPVATAVAPYVSWIRKVTGRSA
Enzyme Length 257
Uniprot Accession Number P51124
Absorption
Active Site ACT_SITE 66; /note=Charge relay system; /evidence=ECO:0000269|PubMed:19542453; ACT_SITE 111; /note=Charge relay system; /evidence=ECO:0000269|PubMed:19542453; ACT_SITE 207; /note=Charge relay system; /evidence=ECO:0000269|PubMed:19542453
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.21.-
Enzyme Function FUNCTION: Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells. {ECO:0000269|PubMed:18523284, ECO:0000269|PubMed:20406824}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Beta strand (14); Chain (1); Disulfide bond (4); Domain (1); Glycosylation (1); Helix (5); Natural variant (1); Propeptide (1); Signal peptide (1); Turn (3)
Keywords 3D-structure;Apoptosis;Cytolysis;Disulfide bond;Glycoprotein;Hydrolase;Immunity;Innate immunity;Protease;Reference proteome;Secreted;Serine protease;Signal;Zymogen
Interact With Q6NX45
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted. Cytoplasmic granule. Note=Granules of large granular lymphocytes.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255
Structure 3D X-ray crystallography (4)
Cross Reference PDB 2ZGC; 2ZGH; 2ZGJ; 2ZKS;
Mapped Pubmed ID 12506019; 15028722; 15494398; 16818775; 19103589; 19896187; 20107908; 21059895; 21857942; 21909423; 21979465; 23099853; 24316590; 27133719; 27441655; 279011; 3052276; 32282833; 33953729; 809512; 82604;
Motif
Gene Encoded By
Mass 27,545
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.4.21.78;3.4.21.B2;