Detail Information for IndEnz0002002456
IED ID IndEnz0002002456
Enzyme Type ID protease002456
Protein Name ATP-dependent protease ATPase subunit HslU
Unfoldase HslU
Gene Name hslU Mchl_0656
Organism Methylorubrum extorquens (strain CM4 / NCIMB 13688) (Methylobacterium extorquens)
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Alphaproteobacteria Hyphomicrobiales Methylobacteriaceae Methylorubrum Methylorubrum extorquens (Methylobacterium dichloromethanicum) (Methylobacterium extorquens) Methylorubrum extorquens (strain CM4 / NCIMB 13688) (Methylobacterium extorquens)
Enzyme Sequence MTTFSPREIVSELDRFIVGQKDAKRAVAIALRNRWRRQQLKGPLRDEVAPKNILMIGPTGCGKTEIARRLARLAGAPFLKIEATKFTEVGYVGRDVEQIVRDLVEVGIGLTREEKRKAVKAKAEAAAESRILDALVGPTASQATRESFRKKLRASELDDKEVEIELAPSGSQGMPMFEIPGMPGASMGAINISDMLGKALGGQRGKPRRITVAEAYAPLIAEESDKLVDDDALTREAIREVEDNGIVFLDEIDKICAREGRSGADVSREGVQRDLLPLIEGTTVATKHGPVKTDHILFIASGAFHVSKPSDLLPELQGRLPIRVELQPLTVEDFKQILTATEASLIKQTVALMETEGVTLDFTEDAIDALARVAVEVNGSVENIGARRLQTVLERVIDEISFTATDRSGETVPIDAAYVRDRVEDLAANADLSRFIL
Enzyme Length 437
Uniprot Accession Number B7KZ48
Absorption
Active Site
Activity Regulation
Binding Site BINDING 18; /note=ATP; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00249; BINDING 250; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00249; BINDING 315; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00249; BINDING 387; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00249
Calcium Binding
catalytic Activity
DNA Binding
EC Number
Enzyme Function FUNCTION: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis. {ECO:0000255|HAMAP-Rule:MF_00249}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding NP_BIND 60..65; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00249
Features Binding site (4); Chain (1); Nucleotide binding (1)
Keywords ATP-binding;Chaperone;Cytoplasm;Nucleotide-binding;Stress response
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00249}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 47,710
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda